Transcriptomics-Based Liquid Biopsy for Early Detection of Recurrence in Locally Advanced Gastric Cancer

被引：6

作者：

Ding, Ping'an ^{[1
,2
,3
]}

Wu, Jiaxiang ^{[1
,2
,3
]}

Wu, Haotian ^{[1
,2
,3
]}

Li, Tongkun ^{[1
,2
,3
]}

Niu, Xiaoman ^{[1
,2
,3
]}

Yang, Peigang ^{[1
,2
,3
]}

Guo, Honghai ^{[1
,2
,3
]}

Tian, Yuan ^{[1
,2
,3
]}

He, Jinchen ^{[1
,2
,3
]}

Yang, Jiaxuan ^{[1
,2
,3
]}

Gu, Renjun ^{[4
,5
,6
]}

Zhang, Lilong ^{[7
]}

Meng, Ning ^{[8
]}

Li, Xiaolong ^{[9
]}

Guo, Zhenjiang ^{[10
]}

Meng, Lingjiao ^{[11
,12
]}

Zhao, Qun ^{[1
,2
,3
]}

机构：

[1] Hebei Med Univ, Hosp 4, Dept Surg 3, Shijiazhuang 050011, Peoples R China

[2] Hebei Key Lab Precis Diag & Comprehens Treatment G, Shijiazhuang 050011, Peoples R China

[3] Big Data Anal & Min Applicat Precise Diag & Treatm, Shijiazhuang 050011, Peoples R China

[4] Nanjing Univ Chinese Med, Sch Chinese Med, Nanjing 210023, Jiangsu, Peoples R China

[5] Nanjing Univ Chinese Med, Sch Integrated Chinese & Western Med, Nanjing 210023, Jiangsu, Peoples R China

[6] Nanjing Univ, Jinling Hosp, Med Sch, Dept Gastroenterol & Hepatol, Nanjing 210002, Jiangsu, Peoples R China

[7] Wuhan Univ, Renmin Hosp, Dept Gen Surg, Wuhan 430065, Hubei, Peoples R China

[8] Shijiazhuang Peoples Hosp, Dept Gen Surg, Shijiazhuang 050050, Hebei, Peoples R China

[9] Baoding Cent Hosp, Dept Gen Surg, Baoding 071030, Hebei, Peoples R China

[10] Hengshui Peoples Hosp, Dept Gen Surg, Hengshui 053099, Hebei, Peoples R China

[11] Hebei Med Univ, Hosp 4, Tumor Res Inst, Shijiazhuang 050011, Peoples R China

[12] Hebei Med Univ, Hosp 4, Res Ctr, Shijiazhuang 050011, Peoples R China

来源：

ADVANCED SCIENCE | 2024年 / 11卷 / 47期

关键词：

gastric cancer; liquid biopsy; mRNA Panel; recurrence detection; transcriptomics; CELL-FREE DNA; METHYLATION; EXPRESSION; DIAGNOSIS; PET/CT; GENES; CEA;

D O I：

10.1002/advs.202406276

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The study presents a transcriptomics-based liquid biopsy approach for early recurrence detection in locally advanced gastric cancer (LAGC). Four mRNA biomarkers (AGTR1, DNER, EPHA7, and SUSD5) linked to recurrence are identified through transcriptomic data analysis. A Risk Stratification Assessment (RSA) model combining these biomarkers with clinical features showed superior predictive accuracy for postoperative recurrence, with AUCs of 0.919 and 0.935 in surgical and liquid biopsy validation cohorts, respectively. Functional studies using human gastric cancer cell lines AGS and HGC-27 demonstrated that silencing the identified mRNA panel genes impaired cell migration, invasion, and proliferation. In vivo experiments further showed reduced tumor growth, metastasis, and lymphangiogenesis in mice, possibly mediated by the cAMP signaling pathway. This non-invasive approach offers significant potential for enhancing recurrence detection and enabling personalized treatment strategies, thereby improving patient outcomes in the management of LAGC.

引用

页数：19

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