A simple method to remove polyimide coating from fused silica capillaries for capillary electrophoresis

被引:0
作者
Wang, Pan [1 ]
Cheng, Yulin [1 ]
Wei, Wei [1 ]
机构
[1] Tianjin Univ Technol, Inst Funct Crystal, Tianjin Key Lab Funct Crystal Mat, Tianjin 300384, Peoples R China
关键词
Capillary electrophoresis; Detection windows; Fused silica capillaries; Polyimide coating; Laser engraving; BRIGHT RED FLUORESCENCE; LARGE STOKES SHIFTS; EXCITED-STATE;
D O I
10.1016/j.chroma.2024.465395
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple and cost-effective method for making capillary electrophoresis (CE) detection windows is reported. A low-cost laser engraving machine equipped with a 450 nm high-power blue laser was used to remove the polyimide coating from the fused silica capillary by laser scanning the desired section. The entire process is automated and controlled in real time by software. This method can be easily adopted by many CE users for routine practice and is scalable for high-throughput production of detection windows across multiple capillaries.
引用
收藏
页数:5
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