Effect of Buffalo Milk Exosomes on Lipid Metabolism Disorder in Liver of Mouse Induced by High-fat-diet

被引：0
作者：
Liang, Huanjie [1 ]
Yu, Jiahan [1 ]
Yang, Zekun [1 ]
He, Mingjin [1 ]
Liu, Qiaoling [1 ]
Lan, Jiahui [1 ]
Zhang, Haojie [1 ,2 ]
机构：
[1] College of Animal Science and Technology, Guangxi University, Nanning
[2] College of Ocean and Fishery, Guangdong Eco-Engineering Polytechnic, Guangzhou
来源：
Science and Technology of Food Industry | 2024年 / 45卷 / 22期
关键词：
buffalo milk; exosomes; high-fat diet; lipid disorder; liver;
D O I：
10.13386/j.issn1002-0306.2023070247
中图分类号：
学科分类号：
摘要：
To investigate the effects of buffalo milk exosomes on lipid metabolism disorders in the liver of mice induced by high-fat diet. In the experiment, exosomes were obtained through ultra-high speed centrifugation from buffalo milk, and a total 32 male C57BL/J6 mice were selected and randomly divided into normal control group (ND group), high-fat diet group (HFD group), low-dose exosomes group (HFD-L group), and high-dose exosomes group (HFD-H group). The mice in normal control group and the other groups were respectively fed with a 10% kcal control feed and a 60% kcal high-fat diet for 8 weeks. Subsequently, the mice were gavaged with either PBS or buffalo milk exosomes (5 and 20 mg/kg) every 48 h for a duration of 28 d, based on their respective groupings. The following indexes were detected: body weight, total adipose, liver tissue masses, levels of serum, liver triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), as well as serum gluten transaminase (AST) and alanine transaminase (ALT). Furthermore, liver tissue was subjected to HE staining and oil red O staining. The results showed that the intact buffalo milk exosomes could be obtained through ultra-high speed centrifugation, and buffalo milk exosomes could alleviate the weight gain caused by a high-fat diet in mice to a certain degree. Additionally, the exosomes significantly reduced liver and serum levels of TG, TC and LDL-C in a dose-dependent manner in high-fat mice, conversely increased liver and serum levels of HDL-C, and increased the serum levels of AST and ALT (P<0.05), slowing down the liver damage in high-fat mice. In addition, the results from both HE staining and oil red O staining demonstrated that buffalo milk exosomes could improve liver vacuolar degeneration and reduce liver lipid deposition in mice (P<0.05). In summary, the buffalo milk exosomes would possess the potential to alleviate liver lipid disorders induced by a high-fat diet, thereby provide a theoretical basis for the application and advancement of milk-derived exosomes as natural nanomedicines. © The Author(s) 2024.
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页码：297 / 304
页数：7
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