Protective Effect of Chitosan Oligosaccharide on Ethanol Induced Inflammatory Reaction

被引：0
作者：
Wang, Bin [1 ,2 ]
Zhao, Yuke [1 ]
Jiang, Qixing [1 ,2 ]
Xu, Yanshun [1 ,2 ]
Xia, Wenshui [1 ,2 ]
机构：
[1] School of Food Science and Technology, Jiangnan University, Jiangsu, Wuxi
[2] Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangsu, Wuxi
来源：
Journal of Chinese Institute of Food Science and Technology | 2024年 / 24卷 / 08期
关键词：
alcohol; antiinflammatory; chitosan oligosaccharide; hippocampus;
D O I：
10.16429/j.1009-7848.2024.08.021
中图分类号：
学科分类号：
摘要：
To study the protective effect of chitosan oligosaccharide on alcohol-induced hippocampal tissue， 39 newborn SD rats were randomly divided into three groups: Blank control group (CON group)， model group (ETOH group) and chitosan oligosaccharide intervention group (COS group). The alcohol injury model of newborn SD rats was established. The COS group received chitosan oligosaccharide intervention. At the end of the intervention on the 9th day after birth (PD9)， no intervention was conducted between PD9 and PD24. The phosphatidylinositol-4，5-bisphosphoinositol-3-kinase (PI3K)/protein kinase B (Akt) of PD9 and PD24 were observed respectively expression of glial fibrillary acidic protein (GFAP) and apoptosis-inducing factor1 (AIF1)， inflammatory factors interleukin 6 (IL-6)， interleukin 10 (IL-10)， tumor necrosis factor α (tumor necrosis factor alpha， TNF-α). The new object recognition experiment is carried out at PD24. The results showed that at PD9， PI3K， Akt proteins and their phosphorylation levels， GFAP and AIF1 protein in COS group were not significantly different from those in ETOH group， but IL-6 and IL-10 were significantly increased， TNF- α significantly decreased. At PD24， compared with ETOH group， the phosphorylation level of PI3K and Akt protein in COS group increased significantly， the expression of GFAP decreased， IL-6 and IL-10 increased significantly， and TNF- α significantly decreased. Chitosan oligosaccharide can alleviate the inhibition of PI3K/Akt pathway and inhibit the overexpression of astrocyte marker GFAP in PD24. © 2024 Chinese Institute of Food Science and Technology. All rights reserved.
引用
收藏
页码：216 / 224
页数：8
相关论文
共 29 条
  • [11] The National Health and Family Planning Commission approved six new food raw materials including chitosan oligosaccharide
  • [12] KELLY S J, LAWRENCE C R., Intragastric intubation of alcohol during the perinatal period, Methods in Molecular Biology, 447, 4, pp. 101-110, (2008)
  • [13] BOBOT M, THOMAS L, MOYON A, Et al., Uremic toxic blood-brain barrier disruption mediated by AhR activation leads to cognitive impairment during experimental renal dysfunction, Journal of the American Society of Nephrology, 31, 7, pp. 1509-1521, (2020)
  • [14] ROCHA R, ANDRADE L, ALVES T, Et al., Behavioral and brain morphological analysis of non-inflammatory and inflammatory rat models of preterm brain injury, Neurobiology of Learning and Memory, 185, 1, (2021)
  • [15] ALMEIDA-TOLEDANO L, ANDREU-FERNANDEZ V, ARAS-LOPEZ R, Et al., Epigallocatechin gallate ameliorates the effects of prenatal alcohol exposure in a fetal alcohol spectrum disorder-like mouse model, International Journal of Molecular Sciences, 22, 2, (2021)
  • [16] WU W, WEI W, LU M, Et al., Neuroprotective effect of chitosan oligosaccharide on hypoxic-ischemic brain damage in neonatal rats, Neurochemical Research, 42, 11, pp. 3186-3198, (2017)
  • [17] MARSHALL S A, GEIL C R, NIXON K., Prior binge ethanol exposure potentiates the microglial response in a model of alcohol-induced neurodegeneration, Brain Sciences, 6, 2, (2016)
  • [18] MCCLAIN J A, MORRIS S A, DEENY M A, Et al., Adolescent binge alcohol exposure induces long-lasting partial activation of microglia, Brain, Behavior, and Immunity, 25, pp. S120-S128, (2011)
  • [19] GONZALEZ A, PARIENTE J A, SALIDO G M., Ethanol stimulates ROS generation by mitochondria through Ca2+ mobilization and increases GFAP content in rat hippocampal astrocytes, Brain Research, 8, 48, pp. 28-37, (2007)
  • [20] WALTER T J, CREWS F T., Microglial depletion alters the brain neuroimmune response to acute binge ethanol withdrawal, Journal of Neuroinflammation, 14, 1, (2017)
123