The Increased Apoptosis of Mesenchymal Stem Cells Mediated Osteopenia Due to Prenatal Nicotine Exposure in Female Offspring Rats via IGF1 Pathway

被引：0
作者：
Li, Xufeng [1 ]
Xiao, Hao [1 ,2 ,3 ]
Wu, Zhixin [1 ]
Wang, Hui [2 ,4 ]
Chen, Liaobin [1 ,2 ,3 ]
机构：
[1] Division of Joint Surgery and Sports Medicine, Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Hubei, Wuhan
[2] Hubei Provincial Key Laboratory of Developmental Originated Disease, Wuhan University, Hubei, Wuhan
[3] Joint Disease Research Center of Wuhan University, Zhongnan Hospital of Wuhan University, Hubei, Wuhan
[4] Department of Pharmacology, Wuhan University School of Basic Medical Sciences, Wuhan University, Hubei, Wuhan
来源：
Environmental Toxicology | 2024年 / 39卷 / 11期
基金：
中国国家自然科学基金;
关键词：
apoptosis; bone developmental toxicity; insulin-like growth factor l; prenatal nicotine exposure;
D O I：
10.1002/tox.24391
中图分类号：
学科分类号：
摘要：
Nicotine exposure is a common adverse environment during pregnancy and causes developmental toxicity of long bones in offspring. However, the effect of prenatal nicotine exposure (PNE) on bone mass accumulation in female offspring and its mechanism remained to be further investigated. In this study, we constructed a PNE rat model and collected the long bone and the bone marrow mesenchymal stem cells (BMSCs) from female offspring rats for the detection of bone mass, cell apoptosis, and the expressions of osteogenesis- and apoptosis-related genes. The results revealed that PNE induced low bone mass in female offspring rats and was associated with the suppression of osteogenic function. Moreover, the apoptosis of BMSCs derived from the PNE female offspring rats was raised, and the expression ratio of apoptosis marker genes BAX/BCL-2 was significantly increased. Further, PNE inhibited the expression and function of insulin-like growth factor l (IGF1) signaling pathway in BMSCs. However, the exogenous IGF1 treatment partially ameliorated the increased apoptosis of BMSCs derived from the PNE female offspring rats. In conclusion, PNE induced low bone mass in female offspring rats, which was attributed to the increased apoptosis of BMSCs due to functional inhibition of IGF1 signaling pathway. © 2024 Wiley Periodicals LLC.
引用
收藏
页码：5199 / 5208
页数：9
相关论文
共 39 条
  • [1] Cui Y., Shooshtari S., Forget E.L., Clara I., Cheung K.F., Smoking During Pregnancy: Findings From the 2009–2010 Canadian Community Health Survey, PLoS One, 9, 1, (2014)
  • [2] Tong V.T., Et al., Trends in Smoking Before, During, and After Pregnancy - Pregnancy Risk Assessment Monitoring System, United States, 40 Sites, 2000-2010, MMWR Surveillance Summaries, 62, 6, pp. 1-19, (2013)
  • [3] Contal M., Et al., Neonatal Consequences of Maternal Smoking During Pregnancy, Journal de Gynecologie, Obstetrique et Biologie de la Reproduction, 34, 1, pp. 3S215-3S222, (2005)
  • [4] Pereira P.P., Et al., Maternal Active Smoking During Pregnancy and low Birth Weight in the Americas: A Systematic Review and Meta-Analysis, Nicotine & Tobacco Research, 19, 5, pp. 497-505, (2017)
  • [5] George L., Granath F., Johansson A.L.V., Anneren G., Cnattingius S., Environmental Tobacco Smoke and Risk of Spontaneous Abortion, Epidemiology, 17, 5, pp. 500-505, (2006)
  • [6] Ion R., Bernal A.L., Smoking and Preterm Birth, Reproductive Sciences, 22, 8, pp. 918-926, (2015)
  • [7] Marufu T.C., Ahankari A., Coleman T., Lewis S., Maternal Smoking and the Risk of Still Birth: Systematic Review and Meta-Analysis, BMC Public Health, 15, (2015)
  • [8] Talati A., Wickramaratne P.J., Wesselhoeft R., Weissman M.M., Prenatal Tobacco Exposure, Birthweight, and Offspring Psychopathology, Psychiatry Research, 252, pp. 346-352, (2017)
  • [9] Machaalani R., Ghazavi E., Hinton T., Waters K.A., Hennessy A., Cigarette Smoking During Pregnancy Regulates the Expression of Specific Nicotinic Acetylcholine Receptor (nAChR) subunits in the Human Placenta, Toxicology and Applied Pharmacology, 276, 3, pp. 204-212, (2014)
  • [10] Deng Y., Gao H., Wang H., Chen L., Snail/HDAC1/2 Mediate Skeletal Growth Retardation in Fetuses Caused by Prenatal Nicotine Exposure, Toxicology, 459, (2021)
1234