Decreased Amino Acid Transporter LAT2 Is the Main Determinant of Impaired Protein Utilization During Aging

被引：0

作者：

Song, Rui ^{[1
]}

Li, Guang ^{[2
]}

Zhao, Liang ^{[1
]}

Qiu, Lili ^{[1
]}

Qin, Xiyu ^{[1
]}

Zhang, Xiaoxu ^{[1
]}

Liu, Xiaoxue ^{[1
]}

Zhou, Jun ^{[1
]}

Hu, Mengxiao ^{[1
]}

Zhang, Liwei ^{[1
]}

Su, Jiaqi ^{[3
]}

Liu, Xinjuan ^{[2
]}

Wang, Xiaoyu ^{[1
]}

机构：

[1] China Agr Univ, Dept Nutr & Hlth, Beijing 100083, Peoples R China

[2] Capital Med Univ, Beijing Chao Yang Hosp, Dept Gastroenterol, Beijing 100069, Peoples R China

[3] Swiss Fed Inst Technol, Dept Hlth Sci & Technol, CH-8092 Zurich, Switzerland

来源：

ENGINEERING | 2024年 / 42卷

关键词：

Aging; Protein utilization; Peptide; Amino acid; Large neutral amino acid transporter 2; ELDERLY-PATIENTS; MEAT PROTEINS; OLDER-ADULTS; DIGESTION; SECRETION; NUTRITION; PRODUCTS; PEPSIN; AGE;

D O I：

10.1016/j.eng.2024.08.009

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

As the global demographic shifts toward an aging population, understanding the efficiency of protein utilization in older adults becomes crucial. Our study explores the intricate relationship between protein intake and aging, with a focus on precision nutrition for older people. Through a meta-analysis, we confirm a decline in protein-utilization capacity in older individuals and examine the different contributions of plant and animal protein. In experiments involving mice of different ages, older mice exhibited decreases in the biological utilization of four proteins (casein, beef protein, soy protein, and gluten), particularly casein. In subsequent research, casein was studied as a key protein. A decline in gastric digestion function was observed through peptidomics and the examination of pepsin levels using casein. Nevertheless, this decline did not significantly affect the overall protein digestion during the aging process. The combined application of targeted amino acid metabolomics identified abnormal absorption of amino acids as the underlying cause of decreased protein utilization during aging, particularly emphasizing a reduction in branched-chain amino acids (BCAAs) in older mice. Delving deeper into the proteomics of the intestinal protein digestion and absorption pathway, a reduction of over 60% in large neutral amino acid transporter 2 (LAT2) protein expression was observed in both older humans and aged mice. The reduction in LAT2 protein was found to be a key factor influencing the diminished BCAA availability. Overall, our study establishes the significance of amino acid absorption through LAT2 in protein utilization during aging and offers a new theoretical foundation for improving protein utilization in the older adults. (c) 2024 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

引用

页码：88 / 98

页数：11