The free tocopherol content in whole berries of six sea buckthorn cultivars grown in northeastern Poland and Belorussia was determined with HPLC. The total free tocopherol content in oil from whole berries was 101.4-128.3 mg/100 g of oil. α-Tocopherol was the predominant tocopherol of sea buckthorn berries, and only traces of γtocopherol were detected in the oil. α- and δ-Tocopherols constituted 62.5-67.9% and 32.1-37.5% of total tocopherol, respectively. The total free tocopherol content in oil of sea buckhtorn cv. Nadbaltycka increased during maturation from 40.4 to 109.8 mg/100 of oil. Green berries contained a marked amount of γ-tocopherol, but its content rapidly declined to traces when the color of berries turned from green to olive-yellow.