Supercritical carbon dioxide was used to extract cottonseed flakes with and without ethanol and 2-propanol as co-solvents to test the capability of supercritical carbon dioxide to extract only triglycerides. After the initial extraction, the remaining compounds in the cottonseed were extracted again with the alcohols to produce a gossypol-rich fraction. This gossypol-rich extract was separated into two fractions. The gossypol content of these fractions was examined to investigate the possible relationship of gossypol with nontriglyceride cottonseed lipids.