Electron holographic observation for biological specimens: Electron holography of bio-specimens

被引：10

作者：

Aoyama, K. ^{[1
,2
]}

Ru, Q. ^{[1
]}

机构：

[1] Tonomura Electron Wavefront Project, ERATO, Res. Devmt. Corporation of Japan, PO Box 5, Hatoyama, Saitama 350-03, Japan

[2] Max-Planck-Instilut F. Biochemie, Abt. Molekulare Struklurbiologie, 82152 Martinsried bei München, Germany

来源：

Journal of Microscopy | 1996年 / 182卷 / 03期

关键词：

Electron holography - Electrons - High resolution transmission electron microscopy - Tobacco - Viruses;

D O I：

10.1046/j.1365-2818.1996.133413.x

中图分类号：

学科分类号：

摘要：

Electron holography has been applied to the observation of biological filaments. The technique has some advantages over conventional imaging for observing weak-phase objects such as small unstained biological structures. To avoid artificial structural transformation of the sample owing to the interaction with the supporting film, a holey carbon film was used to support the filaments. A tobacco mosaic virus bridged over a hole was observed as a cylindrical shape: the contrast distribution across the filament represents its actual shape, which is difficult to obtain with conventional transmission electron microscopy. A number of technical limitations which at present prevent high-resolution structure analysis of biological macromolecules by electron holography are discussed in this report.

引用

页码：177 / 185