A rapid screening assay for measuring urinary androsterone and etiocholanolone δ13C (‰) values by gas chromatography/combustion/isotope ratio mass spectrometry

被引：0

作者：

Aguilera, Rodrigo ^{[1
,3
]}

Chapman, Thomas E. ^{[1
]}

Catlin, Don H. ^{[1
,2
]}

机构：

[1] UCLA Olympic Analytical Laboratory, Department of Molecular and Medical Pharmacology, University of California at Los Angeles, 2122 Granville Avenue, Los Angeles, CA 90025-6106, United States

[2] Department of Medicine, University of California at Los Angeles, 2122 Granville Avenue, Los Angeles, CA 90025-6106, United States

[3] UCLA Olympic Analytical Laboratory, 2122 Granville Avenue, Los Angeles, CA 90025-6106, United States

来源：

| 2000年 / John Wiley and Sons Ltd卷 / 14期

关键词：

Body fluids - Gas chromatography - Mass spectrometry - Phase separation - Screening;

D O I：

10.1002/1097-0231(20001215)14:233.0.CO;2-L

中图分类号：

学科分类号：

摘要：

A gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) method is described and validated for measurement of δ13C values of the acetate derivatives of urinary etiocholanolone and androsterone. The analysis was performed with only 2 mL of urine. The sample preparation consisted of deconjugation with β-glucuronidase, solid phase extraction, and derivatization with acetic anhydride and pyridine. The within-assay precision of two quality control (QC) urine samples ranged from 0.5 to 2.1 CV %. The between-assay precision in the same QC urines ranged from 1.7 to 3.4 CV %. Administration of testosterone enanthate to a subject resulted in a 6‰ decrease in δ13C values from -25‰ (baseline) to -31‰. Two weeks after testosterone administration was discontinued, the δ13C values remained abnormally low while the urine testosterone/epitestosterone (T/E) ratio returned to less than 6. This relatively simple method is useful for rapidly screening a large number of urine samples, including those with T/E

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