Scanning Electron Microscopy of Proteoglycans in Metaphyseal Cartilage

被引：0

作者：

Dell' Orbo, Carlo ^{[1
]}

Quacci, Daniela ^{[1
]}

Riva, Alessandro ^{[1
]}

机构：

[1] Department of Cytomorphology, Umwrslty of Caghan, via Porcell 2. Caghan, 1-09124, Italy

来源：

Microscopy | 1992年 / 41卷 / 06期

关键词：

Alcian blues - Cartilage ultrastructure - Cell membrane surface - Crystalline compounds - Long rod - Proteoglycan histochemistry - Proteoglycans - Scanning electron microscope investigation - Three-dimensional networks;

D O I：

10.1093/oxfordjournals.jmicro.a050996

中图分类号：

学科分类号：

摘要：

Alcian blue (AB) was used for scanning electron microscope investigations on metaphyseal cartilage. In the pericellular area a three-dimensional network connecting the cell membrane surface to the lacunar wall is evident. The network is formed by very long rod-like filaments about 50 nm thick. The segments may be interpreted as the proteoglycans (PGs) of the pericellular area. In the pericellular area in hypertrophic and degenerative zones, the rod-like segments are closely connected to chain granules which are the morphological expression of Ca-P non crystalline compounds. The rod-like segments are not at all evident either in glutaraldehyde-osmium fixed fragments or in predigested-(streptococcal hyaluronidase and chondroitinase) AB stained ones. It is concluded thqt AB is a good method to detect the three-dimensional spatial disposition of cartilage PGs. © 1992 Oxford University Press. All rights reserved.

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页码：475 / 479