This study describes a method of measuring elasticity in individual veins and the application of this technique to compare normal and diseased veins. The elasticity product was calculated from measurements of vein circumference using B-mode ultrasound and intravenous pressures. Incremental changes in pressure were effected using a proximal occlusion cuff. Duplex ultrasound scans were performed on each subject and suitable vein segments identified. 33 subjects (54 segments) were examined, 20 normal and 13 varicose vein subjects. The elasticity product was calculated for each vein segment and the results analyzed using a Mann Whitney U Test. The results demonstrated a significant difference (p<0.05) in the elasticity product of normal and varicose veins subjects. Analysis of individual vein segments demonstrated a lower elasticity product in refluxing veins when compared to non-refluxing veins. There was no significant difference in the elasticity product for non-refluxing veins in normal and varicose vein subjects. These results indicate that a reduction in the elasticity product occurs after the onset of reflux, suggesting that deterioration in vein wall function occurs in response to, rather than initiating reflux.