Chromatographic separation and purification of human chorionic gonadotropin

被引：0
|
作者：
Wang, Hairen [1 ]
Xu, Yutai [1 ]
Wang, Haifeng [1 ]
Qian, Chen [1 ]
机构：
[1] Shandong Univ, Jinan, China
来源：
Lizi Jiaohuan Yu Xifu/Ion Exchange and Adsorption | 1994年 / 10卷 / 02期
关键词：
Adsorption - Chromatography - Ion exchange resins - Purification - Separation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The separation and purification of human chorionic gonadotropin (HCG) in the urine sample of early pregnant women by D3520 resin adsorption chromatography was reported. The crude product obtained by DEAE cellulose -23 and DEAE-Sephadex A50 column chromatography showed a high activity of HCG. Further purification of the sample by gel filtration chromatography on a Sephadex G75 column gives a final preparation of 6000-6500 IU/mg. The preparation meets the requirements of the pyrogen test in Chinese Law of Pharmacopoeia.
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页码：153 / 156
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