ENZYME ELECTRODE FOR GLUTAMATE DETERMINATION.

被引：0
|
作者：
Huang, Yuliang
Yan, Deming
Li, Yourong
机构：
来源：
Huadong Huagong Xueyuan xuebao | 1988年 / 14卷 / 03期
关键词：
BIOSENSORS - Microbial Electrodes - ELECTRODES;
D O I：
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学科分类号：
摘要：
Glutamate decarboxylase (L-glutamate decarboxylase, E, C, AS. 505) immobilised in sodium alginate using the bifunctional agent glutaraldehyde is attached to a carbon dioxide-sensing electrode. The enzyme electrodes thus prepared are used in a bath system which is agitated with an electromagnetic stirrer at constant temperature to measure the concentration of L-glutamic acid. The measuring time for each sample is about 10-15 min by static method and about 1 min by dynamic method. The electrode response is linear between 8. 3-6 300 mg Glu/L with high specificity for L-glutamic acid.
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页码：365 / 373
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