In plasma filtration from blood, concentration polarization in the formation, consists of a high particle concentration boundary layer which consists of cells rejected by the membrane forms a reversible secondary membrane which becomes the limiting filtration mechanism. In this condition, the permeate flux becomes approximately independent of transmembrane pressure and is controlled mostly by the shear rate. To study the kinetics and time scales of establishment of these phenomena, the transient response of microporous membrane under stepped charges in transmembrane pressure was investigated. The results confirm that once concentration polarization is established, the system membrane-polarization layer ceases to behave as a porous medium even with increased resistance. These data can explain the permeate increase observed when pressure and flow pulsations at 1 Hz are superimposed on the retentate.