The process of Bragg gratings fabrication in microstructure fibers is described in this paper. Using terms of a 248 nm KrF excimer laser and a phase mask, fiber Bragg gratings (FBGs) are fabricated in grapefruit fibers, which incorporate six large air holes that form an approximately 40 μm air annulus a-round a central silica region of 27 μm in diameter. And in order to enhance the photosensitivity of the fiber, hydrogen doping method is applied in this work. According to the spectrum, a microstructure fiber based FBG with central wavelength of 1556.87 nm and 3 dB bandwidth of 0.24 nm is achieved successfully.