Use of chiral and achiral ion-pairing reagents in combination with cyclodextrins in capillary electrophoresis

The suitability of chiral and achiral acidic ion-pairing reagents to effect chiral separation in capillary electrophoresis in the presence of cyclodextrins (CDs) has been demonstrated previously. This method was applied to a great variety of analytes. Besides alkylsulfonates, alkanoic acids are able to improve chiral resolution in combination with CD. The dependence of the separation on alkyl chain length is described. The use of the cationic ion-pairing reagent quinine, is a powerful tool in influencing enantiomeric separation of acidic and basic analytes. Methods of direct and indirect UV detection are used in quinine containing running buffers. The influences of pH value, quinine and CD concentration on the separation factors are reported. L-Hyoscyamine showed the best capability to improve the enantioseparation of propranolol.