X-ray and neutron reflectivity study of solid-supported lipid membranes prepared by spin coating

被引：0

作者：

Generosi, Johanna ^{[1
]}

Castellano, Carlo ^{[1
]}

Pozzi, Daniela ^{[1
]}

Congiu Castellano, Agostina ^{[1
]}

Felici, Roberto ^{[2
]}

Natali, Francesca ^{[2
]}

Fragneto, Giovanna ^{[3
]}

机构：

[1] Dipartimento di Fisica, Istituto Nazionale per la Fisica della Materia, Università la Sapienza, P.le Aldo Moro 2, 00185 Rome, Italy

[2] Operative Group in Grenoble-Istituto Nazionale per la Fisica della Materia, 6 rue J. Horowitz, 38043 Grenoble Cedex 9, France

[3] Institute Laue-Langevin, 6 rue J. Horowitz, 38042 Grenoble Cedex 9, France

来源：

Journal of Applied Physics | 2004年 / 96卷 / 11期

关键词：

Biological membranes - Deposition - DNA - Neutrons - Reflection - Spin coating - Synchrotron radiation - X rays;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We present a study of x-ray synchrotron radiation and neutron reflectivity on solid-supported lipid membranes prepared by spin coating. This technique has the advantage of allowing the control of the number of lipid layers by varying the deposition parameters. The experiments were performed on the cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP), the neutral lipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), the lipid mixture (DOTAP-DOPC), and the complex (DOTAP-DOPC/DNA) deposited on wafers. Only single neutral lipids or lipid-peptide mixtures were deposited on solid substrate using the spin coating technique and characterized. Results on the structure of the deposited lipid layers indicate that DNA contributes to the order in the lipoplexes. © 2004 American Institute of Physics.

引用

页码：6839 / 6844

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