On-line estimation of viable cells in a hybridoma culture at various DO levels using ATP balancing and redox potential measurement

A comparison of two on-line methods for the estimation of viable cell number in hybridoma cultivation is reported. One method uses an empirical correlation between redox potential and animal cell density, while the other is based on an ATP balance with ATP steady-state assumption. The two methods are compared based on the measurement of oxygen uptake rate which is carried out either by stationary liquid phase balance with surface aeration or by gas balance during bubble aeration with headspace flushing with an inert gas.