Electroosmotic flow and process characteristics of hydroxylapatite electrochromatography

被引：0

作者：

Yin, Gang ^{[1
]}

Li, Chen ^{[1
]}

Zhan, Jin ^{[1
]}

Yuan, Naiju ^{[1
]}

Liu, Zheng ^{[1
]}

机构：

[1] Dept. of Chem. Eng., Tsinghua Univ., Beijing 100084, China

来源：

Huagong Xuebao/Journal of Chemical Industry and Engineering (China) | 2001年 / 52卷 / 08期

关键词：

Adsorption - Chromatographic analysis - Electroosmosis - Electrophoresis - Hydroxyapatite;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A novel kind of preparative electrochromatography, namely hydroxylapatite electrochromatography (HEC), is proposed in which an electric potential is applied to the compartment packed with hydroxylapatite. The effects of electric migration, including the electrophoresis of protein and the electroosmosis of solvent, on the separation performance of HEC are experimentally investigated. Electroosmotic fluxes of membrane, membrane and packed medium are determined and the driving forces by electroosmotic flux of membrane and packed medium are interpreted respectively. An increase in electroosmotic flux is obtained in response to the increase in electric field strength and the decrease in ionic concentration of the buffer. The breakthrough curves obtained in the presence of an electric field show an enhanced adsorption of BSA on hydroxylapatite in terms of available bed volume for adsorption and dynamic adsorption capacity. When the elution of BSA from hydroxylapatite is conducted with the buffer of high salt concentration, the enhancement of mass transfer is mainly contributed by electrophoresis. In this case the suitable selection of polarity of electric field is essential to resolution. When elution is done with the buffer of low salt concentration, the contribution of electroosmotic flux to mass transfer becomes significant. The above results show that the application of electric field to hydroxylapatite chromatography, as shown by HEC, greatly improves its separation throughput and efficiency.

引用

页码：666 / 672