Preparation and Chromatographic Behavior of Continuous Rod Column for Immobilized Metal Affinity Chromatography

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作者：
Wei, Yin-Mao [1 ]
Huang, Xiao-Dong [1 ]
Geng, Xin-Du [1 ]
机构：
[1] Inst. of Modern Separation Science, Northwest University, Xi'an 710069, China
来源：
Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities | 2001年 / 22卷 / 10期
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摘要：
A procedure for the preparation of continuous rod consisting of poly(glycidyl methacrylate-coethylene dimethacrylate) for immobilized metal affinity chromatography (IMAC) is presented. When chelating Cu(II), Zn(II) or Ni(II) on it, the rod displayed a property of IMAC and the selectivity for protein separation was different from that obtained from the naked rod. An abnormal increase in retention times of proteins was found under the condition of either very high pH or very low pH on the metal chelated rods.
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