The Radioprotective Effect against Gamma-Irradiation of Methylene Blue in the Rat with Reference to Serum Enzymes and Pancreatic Protein Fractions Examined by Isoelectric Focussing

被引：3

作者：

Chung, Se O. ^{[1
]}

Nam, Sang Yul ^{[1
]}

机构：

[1] Department of Biology, Graduate School, Kyung Hee University, Seoul,131, Korea, Republic of

来源：

| 1600年 / Oxford University Press卷 / 16期

关键词：

Gamma irradiation - Glutamic-oxaloacetic transaminase - Glutamic-pyruvic transaminase - Isoelectric focussing - Male rats - Methylene Blue - Protein bands - Protein fractions - Sprague-dawley - Whole-body;

D O I：

10.1269/jrr.16.211

中图分类号：

学科分类号：

摘要：

The Sprague-Dawley male rats were given 360 rads of single whole-body gamma-irradiation following an intraperitoneal injection of methylene blue (40 mg/kg). Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), serum amylase, serum lipase, and serum lecithinase A activities, and isoelectric focussing pattern of pancreatic juice proteins were determined at various time intervals after exposure. Methylene blue reduced generally the rise of SGOT, SGPT, amylase, and lipase activities. Methylene blue delayed also the serum lecithinase A fall after exposure. Mean number of protein bands as revealed by isoelectric focussing of pancreatic juice were significantly altered in both the control and the methylene blue-treated group after exposure. Especially methylene blue-treated group showed a marked delay in the decrease in number of protein bands after exposure. The possibility of using the SGOT, the SGPT, the serum amylase, the serum lipase, and the serum lecithinase A levels, and the number of protein bands in isoelectric focussing pattern of pancreatic juice as an early index of radiation injury is suggested. © 1975 Oxford University Press. All rights reserved.

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