A pheromone-binding protein from the cockroach Leucophaea maderae: Cloning, expression and pheromone binding

被引：37

作者：

Rivière, Stéphane ^{[1
]}

Lartigue, Audrey ^{[2
]}

Quennedey, Brigitte ^{[1
]}

Campanacci, Valérie ^{[2
]}

Farine, Jean-Pierre ^{[1
]}

Tegoni, Mariella ^{[2
]}

Cambillau, Christian ^{[2
]}

Brossut, Rémy ^{[1
]}

机构：

[1] Développement-Commun. Chim., UMR-5548-CNRS, Université de Bourgogne, 21000 Dijon

[2] Arch./Fonction Macromolec. B., UMR-6098-CNRS, Universités Aix-Marseille 1/2, 13402 Marseille Cedex 20

来源：

Biochemical Journal | 2003年 / 371卷 / 02期

关键词：

Dictyoptera; Fluorescent-binding assay; Leucophaea madera; Olfaction; Pheromone-binding protein;

D O I：

10.1042/BJ20021877

中图分类号：

学科分类号：

摘要：

Odorant-binding proteins (OBPs) are thought to transport volatile compounds from air to their receptors through the sensillary lymph. In this protein family, the subgroup of pheromone-binding proteins (PBPs) is specifically tuned to the perception of the sexual pheromone. To date, the description of OBPs has been restricted to Endopterygota and Paraneoptera. Their expression in Orthopteroid has been hypothesized, but no evidence of OBP has been produced in this assemblage to date. In the present study, we describe the first OBP from a Dictyopteran insect that belongs to the cockroach Leucophaea maderae. The PBP of L. maderae (PBPLma) shares all the hallmarks of the OBP family and is expressed specifically in the female adult antennae, the sex that perceives the sexual pheromone. The affinity of the recombinant PBPLma produced in the Escherichia coli periplasm for the pheromonal compounds has been tested by displacement of a fiuorophore, 8-anilino-1-naphtalenesulphonic acid (ANS). Our results suggest that two chemically close compounds of the pheromonal blend (3-hydroxy-butan-2-one and butane-2,3-diol) are capable of displacing ANS, whereas two other pheromone components (E-2-octenoic acid and senecioic acid) and other alkyl volatile compounds are not capable of displacing ANS, indicating a certain filtering of binding, which can be correlated with the putative function.

引用

页码：573 / 579

页数：6

共 38 条

[1]

Sirugue D., Comportement Sexuel et Hierarchie chez Leucophaea maderae et Nauphoeta cinerea (Dictyoptera, Blaberidae), (1992)

[2]

Vogt R.G., Kohne A.C., Dubnau J.T., Prestwich G.D., Expression of pheromone binding proteins during antennal development in the gypsy moth Lymantria dispar, J. Neurosci., 9, pp. 3332-3346, (1989)

[3]

Vogt R.G., Prestwich G.D., Lerner M.R., Odorant-binding-protein subfamilies associate with distinct classes of olfactory receptor neurons in insects, J. Neurobiol., 22, pp. 74-84, (1991)

[4]

Krieger J., Raming K., Breer H., Cloning of genomic and complementary DNA encoding insect pheromone binding proteins: Evidence for microdiversity, Biochim. Biophys. Acta, 1088, pp. 277-284, (1991)

[5]

Rogers M.E., Krieger J., Vogt R.G., Antennal SNMPs (sensory neuron membrane proteins) of Lepidoptera define a unique family of invertebrate CD36-like proteins, J. Neurobiol., 49, pp. 47-61, (2001)

[6]

Rogers M.E., Steinbrecht R.A., Vogt R.G., Expression of SNMP-1 in olfactory neurons and sensilla of male and female antennae of the silkmoth Antheraea polyphemus, Cell Tissue Res., 303, pp. 433-446, (2001)

[7]

Vogt R.G., Callahan F.E., Rogers M.E., Dickens J.C., Odorant binding protein diversity and distribution among the insect orders, as indicated by LAP, an OBP-related protein of the true bug Lygus lineolaris (Hemiptera, Heteroptera), Chem. Senses, 24, pp. 481-495, (1999)

[8]

Galindo K., Smith D.P., A large family of divergent Drosophila odorant-binding proteins expressed in gustatory and olfactory sensilla, Genetics, 159, pp. 1059-1072, (2001)

[9]

Graham L.A., Davies P.L., The odorant-binding proteins of Drosophila melanogaster: Annotation and characterization of a divergent gene family, Gene, 292, pp. 43-55, (2002)

[10]

Blessmann H., Walter M.F., Dimitratos S., Woods D., Isolation of cDNA clones encoding putative odorant binding proteins from the antennae of the malaria-transmitting mosquito, Anopheles gambiae, Insect Mol. Biol., 11, pp. 123-132, (2002)

← 1 2 3 4 →