PRODUCTION OF BUTANOL BY CLOSTRIDIUM ACETOBUTYLICUM IN EXTRACTIVE FERMENTATION SYSTEM.
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Ishii, Shigeo
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Nagoya Univ, Dep of Chemical, Engineering, Nagoya, Jpn, Nagoya Univ, Dep of Chemical Engineering, Nagoya, JpnNagoya Univ, Dep of Chemical, Engineering, Nagoya, Jpn, Nagoya Univ, Dep of Chemical Engineering, Nagoya, Jpn
Ishii, Shigeo
[1
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Taya, Masahito
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Nagoya Univ, Dep of Chemical, Engineering, Nagoya, Jpn, Nagoya Univ, Dep of Chemical Engineering, Nagoya, JpnNagoya Univ, Dep of Chemical, Engineering, Nagoya, Jpn, Nagoya Univ, Dep of Chemical Engineering, Nagoya, Jpn
Taya, Masahito
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Kobayashi, Takeshi
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]
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[1] Nagoya Univ, Dep of Chemical, Engineering, Nagoya, Jpn, Nagoya Univ, Dep of Chemical Engineering, Nagoya, Jpn
An extractive fermentation system was developed to prevent end-product inhibition of Clostridium acetobutylicum IAM 19012, which mainly produces butanol and acetone. Butanol exhibited greater toxicity to the microorganism than acetone, and its growth was completely inhibited above 10 kg/m**3 of butanol. As an extracting solvent suitable for acetone-butanol fermentation, oleyl alcohol (cis-9-octadecen-1-ol) and C-20 guerbet alcohol (branched-chain alcohol of carbon number, 20) were selected from among 29 organic compounds, based on their nontoxicity to the microorganism. These two solvents had high partition coefficients for butanol, and could be reused without deterioration.