PREPARATION OF NORMAL AND ISCHEMIC MYOCARDIAL TISSUE FOR SCANNING ELECTRON MICROSCOPY.

Normal and ischemic myocardium was prepared by slicing and cryofracture techniques for examination with the SEM. The cryofractured tissue provided the most useful and consistent surface features of the cell organelles with a minimum of mechanical disorder compared to other methods. These SEM studies suggest that myocardium prepared by cryofracture technique yields information on normal and ischemic cell structure consistent with data obtained by TEM.