Production of glycerol in batch and fed-batch cultivation processes of Candida krusei

The glycerol yield in batch cultivation of Candida krusei was only 0.377 g/g due to by-products formation during the cell growth phase. To limit glucose consumption and suppress the formation of by-products, fed-batch cultures were conducted, and glucose was continuously added at low rates during the cell growth phase while the rest of glucose was added at the start of the production phase. The cell yield, glycerol yield, and final glycerol concentration in fed-batch culture were increased by 0.258 g/g, 0.058 g/g, and 10 g/L, respectively, compared with those in the batch culture.