Improved plant regeneration from suspension-cultured cells of Iris germanica L. `Skating Party'

被引:0
|
作者
Wang, Yuexin
Jeknic, Zoran
Ernst, Richard C.
Chen, Tony H.H.
机构
来源
Hortscience: A Publication of the American Society for Hortcultural Science | 1999年 / 34卷 / 07期
关键词
D O I
暂无
中图分类号
学科分类号
摘要
To improve the efficiency of iris plant regeneration, we tested the influence of several combinations of Kin and NAA in culture media on the induction of morphogenesis and the subsequent development of plantlets. The highest rates of regeneration (67%) were consistently observed in induction media containing 0.5 μM NAA and either 2.5 or 12.5 μM Kin. Developing medium containing 1.25 μM BA was optimal for high regeneration rates and a high percentage of plantlets simultaneously developing shoots and roots. Rooted plantlets were easily acclimatized and transplanted to various soil mixtures, then grown in the greenhouse. Under optimal conditions as many as 8000 plantlets could be regenerated from 1 g of cells in approximately equals 4 months. Chemical names used: kinetin (Kin); 1-naphthaleneacetic acid (NAA); N6-benzyladenine (BA).
引用
收藏
页码:1271 / 1276
相关论文
共 50 条
  • [1] Improved plant regeneration from suspension-cultured cells of Iris germanica L. 'Skating Party'
    Wang, YX
    Jeknic, Z
    Ernst, RC
    Chen, THH
    HORTSCIENCE, 1999, 34 (07) : 1271 - 1276
  • [2] Efficient plant regeneration from suspension-cultured cells of tall bearded Iris
    Wang, YX
    Jeknic, Z
    Ernst, RC
    Chen, THH
    HORTSCIENCE, 1999, 34 (04) : 730 - 735
  • [3] Efficient plant regeneration from suspension-cultured cells of tall bearded Iris
    Wang, Yuexin
    Jeknic, Zoran
    Ernst, Richard C.
    Chen, Tony H.H.
    Hortscience: A Publication of the American Society for Hortcultural Science, 34 (04): : 730 - 735
  • [4] Plant regeneration from suspension culture of Iris germanica
    Shimizu, K
    Nagaike, H
    Yabuya, T
    Adachi, T
    PLANT CELL TISSUE AND ORGAN CULTURE, 1997, 50 (01) : 27 - 31
  • [5] Plant regeneration from protoplasts of Iris germanica L
    Shimizu, K
    Yabuya, T
    Adachi, T
    EUPHYTICA, 1996, 89 (02) : 223 - 227
  • [6] Plant regeneration from suspension cultures of Iris pumila L.
    Jevremovic, S
    Radojevic, LJ
    PROCEEDINGS OF THE TWENTIETH INTERNATIONAL EUCARPIA SYMPOSIUM - SECTION ORNAMENTALS: STRATEGIES FOR NEW ORNAMENTALS II, 2002, (572): : 59 - 65
  • [7] Plant regeneration from suspension culture of Iris germanica1
    K. Shimizu
    H. Nagaike
    T. Yabuya
    T. Adachi
    Plant Cell, Tissue and Organ Culture, 1997, 50 : 27 - 31
  • [8] Plant regeneration from protoplasts isolated from embryogenic suspension cultured cells of Cinnamomum camphora L.
    Du, L
    Bao, MZ
    PLANT CELL REPORTS, 2005, 24 (08) : 462 - 467
  • [9] Plant regeneration from protoplasts isolated from embryogenic suspension cultured cells of Cinnamomum camphora L.
    Li Du
    Manzhu Bao
    Plant Cell Reports, 2005, 24 : 462 - 467
  • [10] PREPARATION OF COATED VESICLES FROM SUSPENSION-CULTURED PLANT-CELLS
    MERSEY, BG
    NEWCOMB, EH
    FOWKE, LC
    JOURNAL OF CELL BIOLOGY, 1982, 95 (02): : A391 - A391