Polypyrrole based amperometric and potentiometric phosphate biosensors: A comparative study

被引：4

作者：

Lawal, Abdulazeez T. ^{[1
]}

Adeloju, Samuel B. ^{[1
]}

机构：

[1] Nano Science and Sensor Technology Research Group, School of Applied Sciences and Engineering, Monash University, Churchill

来源：

Journal of Applied Sciences | 2012年 / 12卷 / 04期

关键词：

Amperometric detection; Biosensor; Phosphate; Polypyrrole; Potentiometric detection;

D O I：

10.3923/jas.2012.315.325

中图分类号：

学科分类号：

摘要：

The preparation of two electrochemical (potentiometric and amperometric) phosphate biosensors is described and compared. Purine Nucleoside Phosphorylase (PNP) and xanthine oxidase (XOD) were co-immobilised via entrapment into polypyrrole (PPy) films by galvanostatic polymerization. Polypyrrole entrapment was achieved with 0.5 M pyrrole by using a polymerisation time of 200 sec and a mole ratio of 1:8 (6.2 U mL-1 XOD: 49.6 U mL-1 PNP) inboth biosensors. Sensitive amperometric measurements were compared with those of potentiometric measurements obtained for PPy-PNP-XOD-Fe(CN)64- biosensors. A minimum detectable concentration of 1.0 μM phosphate and a linear concentration range of 5-20 μM were achieved with potentiometric PPy-PNP-XOD-Fe(CN)64- biosensor. In comparison, a minimum detectable concentration of 10 μM and a linear concentration range of 0.1-1 mM were achieved with amperometric biosensor. The presence of uric and ascorbic acids had the least effect on the performance of the amperometric and potentiometric PPy-PNP-XOD-Fe (CN) 6 4 biosensors, therefore will not have any effect on phosphate measurement in both biosensors at levels normally present in water. © 2012 Asian Network for Scientific Information.

引用

页码：315 / 325

页数：10

共 71 条

[1]

Adeloju S.B., Moline A.N., Fabrication of ultra-thin polypyrrole-glucose oxidase film from supporting electrolyte-free monomer solution for potentiometric biosensing of glucose, Biosens. Bioelect, 16, pp. 133-139, (2001)

[2]

Adeloju S.B.O., Lawal A., Polypyrrole-based bilayer biosensor for potentiometric determination of phosphate in natural waters, Int. J. Environ. Chem, 85, pp. 771-780, (2005)

[3]

Adeloju S.B.O., Lawal A.T., Fabrication of a bilayer potentiometric phosphate biosensor by cross-link immobilization with bovine serum albumin and glutaraldehyde, Anal. Chimi. Acta, 691, pp. 89-94, (2011)

[4]

Akylmaz E., Yorgancia E., Construction of an amperometric pyruvate oxidase enzyme electrode for determination of pyruvate and phosphate, Electrochim. Acta, 52, pp. 7972-7977, (2007)

[5]

Andolina C.M., Morrow J.R., Luminescence resonance energy transfer in heterodinuclear In<sup>III</sup> complexes for sensing biologically relevant anions, Eur. J. Inorgan. Chem, 2011, pp. 154-164, (2011)

[6]

Bai Y., Tong J.H., Bian C., Xia S.H., Fabrication and characterization of cobalt nanostructure-based microelectrodes for phosphate detection, Key Eng. Mater, 483, pp. 559-564, (2011)

[7]

Bello M., Gonzalez A., Determination of phosphate in cola beverages using nonsuppressed ion chromatography: An experiment introducing ion chromatography for quantitative analysis, J. Chem. Educ, 73, pp. 1174-1176, (1996)

[8]

Berger H.U., Grass I.M., Method of Enzymic Analysis, (1989)

[9]

Cardemil C.V., Smulski D.R., Larossa R.A., Vollmer A.C., Biolum inescent Escherichia coli strains for the quantitative detection of phosphate and ammonia in coastal and suburban watersheds, DNA Celi Biol, 29, pp. 519-531, (2010)

[10]

Chaniotakis N.A., Jurkschat K., Ruhlemann A., Potentiometric phosphate selective electrode based on a multidendate-tin(IV) carrier, Anal. Chim. Acta, 282, pp. 345-352, (1993)

← 1 2 3 4 5 6 7 8 →