To investigate the influences of AM fungi (Glomus intraradices and Glomus mosseae) on the resistance of tobacco to bacterial wilt (R. solan), a pot experiment was conducted to study the influences of AM fungi inoculation on the bacterial wilt disease incidence, the activity of peroxidase (POD) related to disease resistance and MDA content. The results showed that comparing with the CK, AM fungi inoculation could promote the growth of tobacco plants, the dry weight of and P content in tobacco shoot and roots, and N content in tobacco shoot increased significantly, and the disease incidence and index of bacterial wilt reduced. After R. solan inoculating, the POD activity of tobacco leaves inoculated with Glomus intraradices and Glomus mosseae increased by 22.9% and 26.9% respectively, compared with the CK (only inoculated with R. solan); meanwhile, MDA content declined; which indicated that AM fungi could promote the resistance of tobacco to bacterial wilt.