In vitro cytotoxicity, genotoxicity and antioxidant potentials of thymol on human blood cells

被引：11
作者：
机构：
[1] Department of Biology, Faculty of Science, Atatürk University, Erzurum
[2] Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum
来源：
Aydn, E. (elanuraydinn@gmail.com) | 1600年 / Bellwether Publishing, Ltd.卷 / 26期
关键词：
cytotoxicity; oxidative effects; thymol;
D O I：
10.1080/10412905.2013.860411
中图分类号：
学科分类号：
摘要：
The aim of this study was to investigate the genetic, oxidative and cytotoxic effects of thymol (THY) in cultured human blood cells (n=5) for the first time. Human blood cells were treated with THY (0-200 mg/L) for 24 and 48 hours, and then cytotoxicity was detected by lactate dehydrogenase (LDH) release and [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay, while DNA damage was also analyzed by micronucleus (MN) assay, sister chromatid exchanges (SCE) assay and 8-oxo-2-deoxyguanosine (8-OH-dG) level. In addition, biochemical parameters (total antioxidant capacity [TAC] and total oxidative stress [TOS]) were examined to determine oxidative effects. In our in vitro test systems, it was observed that THY had no mutagenic effects on human lymphocytes. On the other hand, THY (at 25, 50 and 75 mg/L) treatment caused statistically important (p < 0.05) increases of TAC levels and increases of TOS levels (only at 200 mg/L) on cultured human blood cells. According to the results of LDH assay, THY induced cytotoxicity on cultured human blood cells in a time- and dose-dependent manner, and THY (at concentrations above 100 mg/L) decreased cell viability. These results suggest that THY may be the therapeutic antioxidant potential in different diseases. © 2013 Taylor & Francis.
引用
收藏
页码：133 / 140
页数：7
相关论文
共 75 条
  • [1] Grice H.C., Safety evaluation of butylated hydroxytoluene (BHT) in the liver, lung and gastrointestinal tract, Food Chem. Toxicol., 24, pp. 1127-1130, (1986)
  • [2] Wichi H.P., Enhanced tumor development by butylated hydroxyanisole (BHA) from the prospective of effect on forestomach and oesophageal squamous epithelium, Food Chem. Toxicol., 26, pp. 717-723, (1988)
  • [3] Luo H., Yamamoto Y., Liu Y., Jung J.S., Kahng H.Y., Koh Y.J., Hur J.S., The in vitro antioxidant properties of Chinese highland lichens, J. Microbiol. Biotechnol., 20, pp. 1524-1528, (2010)
  • [4] Hwang H.S., Winkler-Moser J.K., Liu S.X., Structural effect of lignans and sesamol on polymerization of Soybean oil at frying temperature, J. Am. Oil Chem. Soc., 89, pp. 1067-1076, (2012)
  • [5] Choi J.S., Chung H.Y., Jung H.A., Park H.J., Yokozawa T., Comparative evaluation of antioxidant potential of alaternin (2-hydroxyemodin) and emodin, Journal of Agricultural and Food Chemistry, 48, 12, pp. 6347-6351, (2000)
  • [6] Ziech D., Franco R., Georgakilas A.G., Georgakila S., Malamou-Mitsi V., Schoneveld O., Pappa A., Panayiotidis M.I., The role of reactive oxygen species and oxidative stress in environmental carcinogenesis and biomarker development, Chem. Biol. Interact., 188, pp. 334-339, (2010)
  • [7] Gupta-Elera G., Garrett A.R., Robison R.A., Oneill K.L., The role of oxidative stress in prostate cancer, Eur. J. Cancer Prev., 21, pp. 155-162, (2012)
  • [8] Stohs S.J., The role of free radicals in toxicity and disease, J. Basic. Clin. Physiol. Pharm., 6, pp. 205-228, (2011)
  • [9] Chiurchiu V., Maccarrone M., Chronic inflammatory disorders and their redox control: From molecular mechanisms to therapeutic opportunities, Antioxid. Redox. Signal., 15, pp. 2605-2041, (2011)
  • [10] Van De Braak S.A.A.J., Leijten G.C.J.J., Essential Oils and Oleoresins: A Survey in the Netherlands and Other Major Markets in the European Union., (1999)
12345678