Determination of pore size distribution of gel filtration media by low-field NMR

被引：0
|
作者：
Wang, Xiaojun [1 ,2 ]
Liu, Xinli [1 ]
Li, Xiunan [2 ]
Ma, Guanghui [2 ]
Su, Zhiguo [2 ]
机构：
[1] College of Food and Bioengineering, Qilu University of Technology
[2] National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences
来源：
Huagong Xuebao/CIESC Journal | 2013年 / 64卷 / 11期
关键词：
Gel filtration media; ISEC; Low-field NMR; Pore structure; Transverse relaxation time;
D O I：
10.3969/j.issn.0438-1157.2013.11.028
中图分类号：
学科分类号：
摘要：
Gel filtration media play a very important role in separation and purification of bio-macromolecules such as proteins, polysaccharides. Their pore structure directly influences protein transport and adsorption in chromatographic separation column. The pore size distribution (PSD) plays a major role beyond simply the mean pore size. Various methods have been employed to determine the PSD of porous materials, including gas adsorption-desorption (BET), mercury intrusion porosimetry (MIP). Since most of gel filtration media is soft gel, so it is difficult to measure their PSD by conventional method. In this paper, a new method, low-field NMR, was explored to determine the pore size distribution of gel filtration media. First, the distribution range of internal water, external water and free water in transverse relaxation time (T2) of low-field NMR were setting-out; then comparing the results with that from inverse size-exclusion chromatography (ISEC) and finding relationship between the pore size and relaxation time in internal water; finally, the pore size distribution of media was fitted by the Gaussian normal distribution. The results demonstrated that low-field NMR is an effective method for PSD determination of porous chromatographic media. The method is easy to operate, simple and quick, and can provide a reference for other determination of chromatography media pore size distribution. © All Rights Reserved.
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页码：4090 / 4095
页数：5
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