Comparison of specific adsorbents for tumor necrosis factor-α and therapeutic anti-tumor necrosis factor-α antibodies: An in vitro sepsis model

被引：0

作者：

Schwanzer-Pfeiffer, Dagmar ^{[1
]}

Mitteregger, R. ^{[1
]}

Rossmanith, E. ^{[1
]}

Falkenhagen, D. ^{[1
]}

机构：

[1] Christian Doppler Laboratory, Center for Biomedical Technology, Danube University Krems, Dr. Dorrekstr. 30, 3500 Krems, Austria

来源：

International Journal of Artificial Organs | 2006年 / 29卷 / 12期

关键词：

Background: Tumor necrosis factor alpha (TNF-α); as a key mediator; represents a major point of attack in sepsis. Since it has been shown that systemic anti-TNF-α antibodies do not improve the situation of septic patients; the use of specific adsorption technology in the treatment of sepsis could have beneficial effects. Methods: Magnetic beads coated with polyclonal or with monoclonal anti-TNF-α antibodies were investigated in vitro in order to analyze their ability to prevent TNF-α induced adhesion of peripheral blood mononuclear cells (PBMCs) at human umbilical vein endothelial cells (HUVECs). Additionally; therapeutical monoclonal anti-TNF-α antibodies were proofed for inhibitory effects of TNF-α mediated activation of HUVECs. Results: We have shown; in vitro; that beads coated with polyclonal or monoclonal anti-TNF-α antibodies were able to significantly reduce monocyte adhesion. It was possible to decrease monocyte adhesion from nearly 9% to 3% within 2 hours and from 18% to 2% within 6 hours of TNF-α treatment by the simultaneous use of beads coated with polyclonal anti-TNF-α antibodies. Beads coated with monoclonal anti-TNF-α antibodies could even prevent monocyte adhesion within the first 2 hours; and reduced monocyte adhesion to 2% during 6 hours of incubation with TNF-α On the other hand; application of therapeutic anti-TNF-α antibodies showed no significant difference compared to the measured monocyte adhesion values of activated endothelial cells. Conclusion: Adsorption techniques using specific adsorbents; possibly used in MDS (Microspheres-Based Detoxification System); are efficient in specific reduction of TNF-α and pathophysiological consequences; since monocyte adhesion at activated HUVECs was shown to be reduced. © Wichtig Editore; 2006;

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摘要：

Journal article (JA)

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页码：1140 / 1147