RP-HPLC-UV method for simultaneous determination of nine isoflavonoids in Huangqi-Gegen herbal pair

被引：0
作者：
Medical Technology and Engineering College, Henan University of Science and Technology, Luoyang, China [1 ]
不详 [2 ]
不详 [3 ]
不详 [4 ]
机构：
[1] Medical Technology and Engineering College, Henan University of Science and Technology, Luoyang
[2] School of Pharmacy, Guangdong Pharmaceutical University
[3] School of Chinese Materia Media, Guangzhou University of Chinese Medicine, Guangzhou
[4] Institute of Biological Medicine, Jinan University, Guangzhou
来源：
Adv. J. Food Sci. Technol. | / 3卷 / 173-176期
关键词：
Active constituents; HPLC; Huangqi-gegen; Isoflavonoids; Pair medicine;
D O I：
10.19026/ajfst.7.1288
中图分类号：
学科分类号：
摘要：
A new simple and efficient RP-HPLC-UV method was developed for the simultaneous analysis of nine isoflavonoids in Huangqi-Gegen herbal pair. Effective chromatographic separation of these components was achieved on a Kromasil C18 column (4.6×250 mm, i.d.; 5 μm) with gradient elution of methanol and 0.2% formic acid aqueous solution at a flow rate of 1.0 mL/min in 60 min. Detection was performed at 250nm. The method was proved to be linear in the ranges of 81.28-406.4, 670.4-3352, 136.96-684.8, 33.376-166.88, 22.848-114.24, 10.176-50.88, 31.04-155.2, 44.544-222.72 and 26.56-132.8 ng for the nine isoflavonoid: 3'-hydroxypuerarin, puerarin, daidzin, calycosin-7-O-β-D-glucoside, genistin, ononin, daidzein, calycosin and formononetin, respectively. The average recoveries were 99.27, 102.38, 98.46, 103.06, 101.29, 99.71, 102.28, 97.89 and 100.78 % respectively; RSD was 1.79, 2.02, 1.44, 1.37, 1.26, 1.81, 1.29, 0.97 and 1.77 % respectively. The developed method was successfully applied for the quantitative analysis of constituents in huangqi gegen herbal pair. © Maxwell Scientific Organization, 2015.
引用
收藏
页码：173 / 176
页数：3
相关论文
共 19 条
  • [1] Bebrevska L., Fouberta K., Hermansb N., Chatterjeec S., Marckc E.V., Meyerd G.D., Lietincka A., Pietersa L., Apers S., In vivo antioxidative activity of a quantified Pueraria lobata root extract, J. Ethnopharmacol., 127, pp. 112-117, (2010)
  • [2] Chang Q., Sun L., Zhao R.H., Moses S.S.C., Zuo Z., Simultaneous determination of ten active components in traditional Chinese medicinal products containing both Gegen (Pueraria lobata) and Danshen (Salvia miltiorrhiza) by high-performance liquid, Phytochem. Anal., 19, pp. 368-375, (2008)
  • [3] Chen S.B., Yang D.J., Chen S.L., Xu H.X., Albert S.C.C., Seasonal variations in the isoflavonoids of Radix Puerariae, Phytochem. Anal., 18, pp. 245-250, (2007)
  • [4] Fan Y., Wu D.Z., Gong Y.Q., Zhou J.Y., Hu Z.I., Effects of calycosin on the impairment of barrier function induced by hypoxia in human umbilical vein endothelial cells, Eur. J. Pharmacol., 481, pp. 33-40, (2003)
  • [5] Huh J.E., Seo D.M., Baek Y.H., Choi D.Y., Park D.S., Lee J.D., Biphasic positive effect of formononetin on metabolic activity of human normal and osteoarthritic subchondral osteoblasts, Int. Immunopharmacol., 10, pp. 500-507, (2010)
  • [6] Jiang R.W., Lau K.M., Lam H.M., Yam W.S., Leung L.K., Choi K.L., Waye M.M.Y., Mak T.C.W., Woo K.S., Fung K.P., A comparative study on aqueous root extracts of Pueraria thomsonii and Pueraria lobata by antioxidant assay and HPLC fingerprint analysis, J. Ethnopharmacol., 96, pp. 133-138, (2005)
  • [7] Kim J.M., Lee Y.M., Lee G.Y., Jang D.S., Bae K.H., Kim J.S., Constituents of the roots of Pueraria lobata inhibit formation of advanced glycation end products (AGEs), Arch. Pharm. Res., 29, pp. 821-825, (2006)
  • [8] Kim S.H., Heo J.H., Kim Y.S., Kang S.S., Choi J.S., Lee S.M., Protective effect of daidzin against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice, Phytother. Res., 23, pp. 701-706, (2009)
  • [9] Lin C.C., Wu C.I., Sheu S.J., Determination of 12 pueraria components by high-performance liquid chromatography-mass spectrometry, J. Sep. Sci., 28, pp. 1785-1795, (2005)
  • [10] Pan H.P., Li G., Protecting mechanism of puerarin on the brain neurocyte of rat in acute local ischemia brain injury and local cerebral ischemia-reperfusion injury, Yakugaku Zasshi, 128, pp. 1689-1698, (2008)
12