Analysis on death receptor apoptotic pathway of SKOV3 cells induced by nanosecond pulsed electric field

被引：0

作者：

Guo, Fei ^{[1
]}

Yao, Chenguo ^{[1
]}

Wang, Jian ^{[1
]}

Sun, Caixin ^{[1
]}

Xia, Rumin ^{[2
]}

Tang, Junying ^{[2
]}

机构：

[1] State Key Laboratory of Power Transmission Equipment and System Security and New Technology, Chongqing University

[2] First Affiliated Hospital, Chongqing Medical Science University

来源：

Gaodianya Jishu/High Voltage Engineering | 2012年 / 38卷 / 05期

关键词：

Apoptosis; Apoptotic pathway of death receptor; Bioelectric effect; Nanosecond pulsed electric field (nsPEF); SKOV3; cell; Tumor cell;

D O I：

10.3969/j.issn.1003-6520.2012.05.012

中图分类号：

学科分类号：

摘要：

The specific bioelectric effect of tumor cells apoptosis induced by nanosecond pulsed electric field (nsPEF) has aroused great attention. Based on the latest studies, the effects of nsPEF on plasma membrane were illustrated to study the signaling pathway of death receptor apoptotic. Therefore, optimized parameters (voltage amplitude of 9 kV, pulse duration of 100 ns, pulse number of 30, repetition frequency of 1 Hz) of nsPEF were performed on SKOV3 cells. Cell death and apoptosis were tested by flow cytometry, massager ribonucleic acid (mRNA) release of Fas, FasL, cysteine aspartic acid specific protease-8 (Caspase-8) and Bid were examined by reverse transcription-polymerase chain reaction (RT-PCR) method, and protein release of Fas, FasL, Caspase-8 and Bid were studied by western blot technology. Experimental results indicate that release of Fas, FasL, Caspase-8 and Bid greatly increase when tumor cell apoptosis with nsPEF, in advance to trigger the apoptotic signaling pathway of death receptor. The results provide a theoretic support for clinical tumor treatment with boarding the mechanism study of nsPEF-induced apoptosis.

引用

页码：1099 / 1105

页数：6

共 27 条

[1]

Beebe S.J., Fox P.M., Rec L.J., Et al., Nanosecond, high-intensity pulsed electric field induce apoptosis in human cells, The Journal of the Federation of American Societies for Experimental Biology, 17, 11, pp. 1493-1495, (2003)

[2]

Kotnik T., Miklavcic D., Theoretical evaluation of voltage inducement on internal membranes of biological cells exposed to electric fields, Biophysical Journal, 90, 2, pp. 480-491, (2006)

[3]

Esser A.T., Smith K.C., Gowrishankar T.R., Et al., Mechanisms for the intracellular manipulation of organelles by conventional electroporation, Biophysical Journal, 98, 11, pp. 2506-2514, (2010)

[4]

Schoenbach K.H., Beebe S.J., Buescher E.S., Intracellular effect of ultrashort electrical pulses, Bioelectromagnetics, 22, 6, pp. 440-448, (2001)

[5]

Buescher E.S., Schoenbach K.H., Effects of submicrosecond, high intensity pulsed electric fields on living cells-intracellular electromanipulation, IEEE Transactions on Dielectrics and Electrical Insulation, 10, 5, pp. 788-794, (2003)

[6]

White J.A., Blackmore P.F., Schoenbach K.H., Et al., Stimulation of capacitative calcium entry in HL-60 cells by nanosecond pulsed electric fields, The Journal of Biological Chemistry, 279, 22, pp. 22964-22972, (2004)

[7]

Vernier P.T., Sun Y., Marcu L., Et al., Calcium bursts induced by nanosecond electric pulses, Biochemical and Biophysical Research Communications, 310, 2, pp. 286-295, (2003)

[8]

Beebe S.J., Blackmore P.F., White J., Et al., Nanosecond pulsed electric fields modulate cell function through intracellular signal transduction mechanisms, Physiological Measurement, 25, 4, pp. 1077-1093, (2004)

[9]

Beebe S.J., Fox P.M., Rec L.J., Et al., Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibition, IEEE Transactions on Plasma Science, 30, 1, pp. 286-291, (2002)

[10]

Chen X.H., Kolb J.F., Swanson R.J., Et al., Apoptosis initiation and angiogenesis inhibition: Melanoma targets for nanosecond pulsed electric fields, Pigment Cell & Melanoma Research, 23, 4, pp. 554-563, (2010)

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