Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-Nitrophenol

被引：0
作者：
Xu Y. [1 ]
Xin J. [1 ,2 ]
Li H. [1 ]
Wang G. [1 ]
Wang Y. [1 ]
机构：
[1] Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin
[2] State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou
来源：
Shipin Kexue/Food Science | 2023年 / 44卷 / 14期
关键词：
detection; fluorescence inner filter effect; gold nanocluster; lipase activity; optical properties;
D O I：
10.7506/spkx1002-6630-20220815-169
中图分类号：
学科分类号：
摘要：
Lipase activity was detected based on the fluorescence properties of gold nanoclusters (AuNCs) and the inner filter effect (IFE) of p-nitrophenol. Glutathione-modified AuNCs was used as fluorophore, and p-nitrophenol produced from the lipase-catalyzed hydrolysis of p-nitrophenol palmitate as fluorescence absorbent. The results showed that under the optimal conditions (p-nitrophenyl palmitate concentration 1.6 mg/mL, pH 7.5, temperature 50 ℃, and reaction time 20 min), the relative fluorescence intensity (F0–F, y) was positively correlated with lipase activity (x) in the range of 5.6–196 U/L, which was described by the equation y = 2.003 5 + 0.936 8x, with a correlation coefficient (r2) of 0.997 8, and the detection limit was 1.3 U/L (at a signal-to-noise ratio of 3). The developed method is simple and sensitive, and can be applied to the detection of lipase activity. © 2023 Chinese Chamber of Commerce. All rights reserved.
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页码：100 / 105
页数：5
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