The main bridge of the Wusu Bridge in Heixiazi Island is a long cantilever steel box composite girder cable-stayed bridge with a single pylon, a single cable plane and with span arrangement (140+140) m. The stay cables and main girder of the bridge are connected, using the steel anchor box cable-to-girder anchorage structure. To ensure the safety of the structure at the service stage, the finite element software ANSYS was used to set up the model for the single block steel box girder and the stress distribution of the structure under the designed stay cable forces was analyzed. The full-scale model for the structure was fabricated as well and the test study of the fatigue performance of the model was carried out under the condition of 500 kN fatigue load amplitude, using the equivalent method of fatigue accumulative damage and with reference to the designed traffic flow of the bridge and the related foreign codes. The results of the test study indicate that under the action of the fatigue load, the stress of the structure is far less than the allowable values as specified in the codes, the fatigue resistance performance of the structure can satisfy the service requirements and the structure has good durability and sufficient safety margin.