Improved podophyllotoxin production by transformed cultures of Linum album

被引：13

作者：

Plant Cell Culture Laboratory, Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016, India ^{[1
]}

机构：

[1] Plant Cell Culture Laboratory, Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110 016, Hauz Khas

来源：

Biotechnol. J. | 2008年 / 9-10卷 / 1256-1263期

关键词：

Agrobactrium; Hairy root-Linum album; Podophyllotoxin; Transformed culture;

D O I：

10.1002/biot.200800173

中图分类号：

学科分类号：

摘要：

Various cell and hairy root cultures of L. album were developed and analyzed for podophyllotoxin content. Transformed callus and hairy root cultures developed from infection of stem portions of in vitro-germinated L. album plant with Agrobacterium rhizogenes NCIM 5140 strain were selected on the basis of high podophyllotoxin content and growth. Based on the integration of Ri TL-DNA and TR-DNA, integration of only the ags and not the rol gene in transformed cell culture indicated fragmented integration pattern. The effect of different cultivation media and carbon source on growth and podophyllotoxin production were studied in shake-flask suspension cultures. Detailed batch growth and production kinetics with sugar consumption profile were also established. Maximum volumetric productivity of 4.40 and 2.75 mg/L per day was obtained in cell suspension and hairy root cultures, respectively. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1256 / 1263

页数：7

共 25 条

[1]

Checklist of CITES species: A reference to the appendices to the Convention on international trade in endangered species of wild fauna and flora, (2001)

[2]

Hadimani S.B., Tanpure R.P., Bhat S.V., Asymmetric total synthesis of (-) podophyllotoxin, Tetrahedron Lett, 37, pp. 4791-4794, (1996)

[3]

Medarde M., Ramos A.C., Caballero E., Lopez J.L., Et al., A new approach to the synthesis of podophyllotoxin based on epimerization reactions, Tetrahedron Lett, 37, pp. 2663-2666, (1996)

[4]

Baldi A., Bisaria V.S., Srivastava A.K., Biotechnological approaches for production of some plant based chemotherapeutics, Medicinal Plant Biotechnology - From Basic Research to Industrial Applications, pp. 117-156, (2007)

[5]

Peterson M., Alfermann A.W., The production of cytotoxic lignans by plant cell cultures, Appl. Microbiol. Biotechnol, 55, pp. 135-142, (2001)

[6]

Ionkova I., Biotechnological approaches for production of lignans, Phcog. Rev, 1, pp. 57-68, (2007)

[7]

Murashige T., Skoog F., A revised medium for rapid growth and bioassay of tobacco tissue cultures, Physiol. Plant, 15, pp. 473-497, (1962)

[8]

Bhojwani S.S., Rajdan M.K., Tissue culture media, Plant Tissue Culture, Theory and Practice, pp. 39-61, (1996)

[9]

Miller G.M., Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem, 31, pp. 426-428, (1959)

[10]

Dubois M., Gilles K.A., Hamilton J.K., Roberts P.A., Et al., Colorimetric method for determination of sugars and related substances, Anal. Chem, 28, pp. 350-356, (1956)

← 1 2 3 →