Dietary restriction by growth in axenic medium induces discrete changes in the transcriptional output of genes involved in energy metabolism in Caenorhabditis elegans

被引：23

作者：

Castelein, Natascha ^{[1
]}

Hoogewijs, David ^{[1
]}

De Vreese, Annemie ^{[1
]}

Braeckman, Bart P. ^{[1
]}

Vanfleteren, Jacques R. ^{[1
]}

机构：

[1] Department of Biology, Ghent University, 9000 Ghent

来源：

Biotechnology Journal | 2008年 / 3卷 / 06期

关键词：

Caenorhabditis elegans; Dietary restriction; Energy metabolism; Transcription profile;

D O I：

10.1002/biot.200800003

中图分类号：

学科分类号：

摘要：

Dietary restriction increases life span in a wide range of species, including the nematode worm Caenorhabditis elegans. The mechanism by which it does so remains largely unknown, although it is commonly thought that a reduction of reactive oxygen species (ROS) plays a pivotal role. More specifically, for C. elegans, it has been proposed that food restriction reduces energy expenditure, possibly in conjunction with an anaerobic shift in energy production, with consequent reduction in the formation of ROS. We have measured differential transcript abundance of 49 genes known to play roles in energy metabolism in axenic culture medium, which causes a nutritional deficit and leads to a substantial increase of life span. We found no evidence for a reduction in metabolic rate or a shift to anaerobic metabolism in axenic culture. Major changes induced by growth in axenic medium include down-regulation of lipid degradation and up-regulation of glyoxylate cycle activity glyceroneogenesis and, possibly, gluconeogenesis. The activities determined in worm extracts for pyruvate kinase, phosphoenolpyruvate carboxykinase and isocitrate lyase followed a similar trend. We conclude that growth in axenic culture is marked by a general up-regulation of replenishing pathways. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：803 / 812

页数：9

共 38 条

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