Separation and purification of isopsoralen and psoralen from Psoralea corylifolia L. by supercritical fluid chromatography

被引：0

作者：

Li F. ^{[1
]}

Kang J.-J. ^{[1
]}

Li A.-F. ^{[1
]}

Sun A.-L. ^{[1
]}

Liu R.-M. ^{[1
]}

机构：

[1] College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng

来源：

Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | 2019年 / 33卷 / 06期

关键词：

Isopsoralen; Psoralea corylifolia L; Psoralen; Supercritical fluid chromatography; Thermodynamics;

D O I：

10.3969/j.issn.1003-9015.2019.06.019

中图分类号：

学科分类号：

摘要：

A supercritical fluid chromatography (SFC) method was established for the separation and purification of isopsoralen and psoralen from Psoralea corylifolia L. The analytical conditions were: three columns including YMC-C18, YMC-Diol and YMC-NH2, four modifiers including methanol, acetonitrile, ethanol and isopropanol, flow rate of mobile phase 2~6 mL∙min-1, separation temperature 300~325 K and separation pressure 10~14 MPa. Moreover, the conditions for preparative SFC were: column = YMC-Diol, mobile phase = supercritical carbon dioxide containing 2% (v/v) methanol at a flow rate of 14.0 mL∙min-1, separation temperature = 313 K and separation pressure = 12 MPa. The result shows that the purities of isopsoralen and psoralen are both above 98%. The Van't Hoff curve shows that the SFC separation is an enthalpy control process under the experimental conditions. © 2019, Editorial Board of Journal of Chemical Engineering of Chinese Universities". All right reserved."

引用

页码：1437 / 1443

页数：6