Separation and purification of isopsoralen and psoralen from Psoralea corylifolia L. by supercritical fluid chromatography

被引:0
作者
Li F. [1 ]
Kang J.-J. [1 ]
Li A.-F. [1 ]
Sun A.-L. [1 ]
Liu R.-M. [1 ]
机构
[1] College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng
来源
Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | 2019年 / 33卷 / 06期
关键词
Isopsoralen; Psoralea corylifolia L; Psoralen; Supercritical fluid chromatography; Thermodynamics;
D O I
10.3969/j.issn.1003-9015.2019.06.019
中图分类号
学科分类号
摘要
A supercritical fluid chromatography (SFC) method was established for the separation and purification of isopsoralen and psoralen from Psoralea corylifolia L. The analytical conditions were: three columns including YMC-C18, YMC-Diol and YMC-NH2, four modifiers including methanol, acetonitrile, ethanol and isopropanol, flow rate of mobile phase 2~6 mL∙min-1, separation temperature 300~325 K and separation pressure 10~14 MPa. Moreover, the conditions for preparative SFC were: column = YMC-Diol, mobile phase = supercritical carbon dioxide containing 2% (v/v) methanol at a flow rate of 14.0 mL∙min-1, separation temperature = 313 K and separation pressure = 12 MPa. The result shows that the purities of isopsoralen and psoralen are both above 98%. The Van't Hoff curve shows that the SFC separation is an enthalpy control process under the experimental conditions. © 2019, Editorial Board of Journal of Chemical Engineering of Chinese Universities". All right reserved."
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页码:1437 / 1443
页数:6
相关论文
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