Quantitative Analysis and Antioxidant Activity of Polyphenolic Compounds in Whole Grain, Rice, and Bran of Hulless Barley

被引：0

作者：

Deng, Junlin ^{[1
]}

Xia, Chen ^{[1
]}

Yang, Kaijun ^{[2
]}

Liu, Tinghui ^{[2
]}

Chen, Jian ^{[1
]}

Xie, Shigang ^{[2
]}

Xiang, Zhuoya ^{[1
]}

Yang, Xing ^{[1
]}

Zhu, Boyu ^{[1
]}

Yu, Manyou ^{[1
]}

机构：

[1] Institute of Agri-products Processing Science and Technology, Institute of Food Nutrition and Health, Sichuan Academy of Agricultural Sciences, Chengdu

[2] Ganzi Tibetan Autonomous Prefecture Institute of Agricultural Sciences, Kangding

来源：

Science and Technology of Food Industry | 2024年 / 45卷 / 21期

关键词：

anthocyanin; antioxidant activity; hulless barley; polyphenol; UPLC;

D O I：

10.13386/j.issn1002-0306.2023110217

中图分类号：

学科分类号：

摘要：

This study focused on the extraction and analysis of β-glucan, free and bound phenols, total phenolic content (TPC), and total flavonoid content (TFC) from whole grain, rice, and bran of purple and glutinous hulless barley, and assessment of the antioxidant activity of extracts. Ultra-high-performance liquid chromatography (UPLC) quantified various phenolic compounds, including protocatechuic aicd, p-hydroxybenzoic acid, vanillic acid, caffeic acid, vanillin, pcoumaric acid, ferulic acid, sinspic acid, luteolin-7-O-glucuronide, chrysoeriol-7-O-glucuronide, luteolin, chrysoeriol, anthocyanin and 8 types of ferulic acid dimers. The antioxidant activities of free and bound phenolic extracts were evaluated by using DPPH and ABTS+ free radical scavenging assays. The results revealed that β-glucan content was comparable in whole grains and barley rice but significantly higher than that in bran (P<0.05). TPC and TFC were higher in bran than in whole grains or rice, with free phenolic extracts showing higher content than bound phenolic extracts. Bran contained a greater variety and higher content of bound polyphenols, followed by whole grains and rice. Particularly, ferulic acid was abundant in the bound phenolic extracts of bran of both barley types. The free phenolic extract of purple hulless barley bran showed high levels of chrysoeriol-7-O-glucuronic acid (1780.78 μg/g) and anthocyanins (2137.67 μg/g). Antioxidant assays demonstrated that hulless barley bran exhibited a stronger capability to scavenge DPPH and ABTS+ free radicals than whole grains and rice. This study provides a partial theoretical basis for the development and utilization of whole grain of hulless barley and hulless barley bran. © The Author(s) 2024.

引用

页码：246 / 253

页数：7

共 46 条

[1]

XIE W Z., Medicine llustrated handbook of compendium of materia medica[M], (2001)

[2]

ZHAO B., Research on the key technology of hull-less barley moderate process and stabilization and mechanism for improvement of product quality, (2021)

[3]

ZHANG K, YANG J, QIAO Z, Et al., Assessment of β-glucans, phenols, flavor and volatile profiles of hulless barley wine originating from highland areas of China[J], Food Chemistry, 293, pp. 32-40, (2019)

[4]

DENG N, ZHENG B S, LI T, Et al., Assessment of the phenolic profiles, hypoglycemic activity, and molecular mechanism of different highland barley （Hordeum vulgare L.） varieties[J], International Journal of Molecular Sciences, 21, 4, (2020)

[5]

GUO T L, HORVATH C, CHEN L, Et al., Understanding the nutrient composition and nutritional functions of highland barley （Qingke）：A review[J], Trends in Food Science and Technology, 103, pp. 109-117, (2020)

[6]

Whole grain cereals：Functional components and health benefits[J], Food and Function, 3, 2, pp. 110-119, (2012)

[7]

VAN HUNG P., Phenolic compounds of cereals and their antioxidant capacity[J], Critical Reviews in Food Science and Nutrition, 56, 1, pp. 25-35, (2016)

[8]

LIU R H., Whole grain phytochemicals and health[J], Journal of Cereal Science, 46, 3, (2007)

[9]

XIA C, YANG K, ZHU Y, Et al., Distribution of free and bound phenolic compounds, β-glucan, and araboxylan in fractions of milled hulless barley[J], LWT, 169, (2022)

[10]

WEN Y., Studies on antilipemic function of β-glucan from highland barley

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