Effect of simulated microgravity on oxidation-sensitive gene expression in PC12 cells

被引：5

作者：

Department of Mechanical Engineering, University of Cincinnati, Cincinnati, OH 45221, United States ^{[1
]}

不详 ^{[2
]}

不详 ^{[3
]}

不详 ^{[4
]}

不详 ^{[5
]}

不详 ^{[6
]}

机构：

[1] Department of Mechanical Engineering, University of Cincinnati, Cincinnati

[2] Department of Environmental Health, Center for Environmental Genetics, Division of Biostatistics and Epidemiology, Cincinnati

[3] Department of Environmental Health, Center for Environmental Genetics, Division of Environmental Genetics and Molecular Toxicology, Cincinnati

[4] Department of Pharmacology and Cell Biophysics, University of Cincinnati, Cincinnati

[5] Microgravity Science Division, NASA Glenn Research Center, Cleveland

[6] Department of Biomedical Engineering, University of Cincinnati, Cincinnati

来源：

Adv. Space Res. | 2006年 / 6卷 / 1168-1176期

关键词：

Gene expression; Microarray; Rotating wall vessel; Simulated microgravity;

D O I：

10.1016/j.asr.2006.02.059

中图分类号：

学科分类号：

摘要：

Oxygen utilization by and oxygen dependence of cellular processes may be different in biological systems that are exposed to microgravity (micro-g). A baseline in which cellular changes in oxygen sensitive molecular processes occur during micro-g conditions would be important to pursue this question. The objective of this research is to analyze oxidation-sensitive gene expression in a model cell line [rat pheochromocytoma (PC12)] under simulated micro-g conditions. The PC12 cell line is well characterized in its response to oxygen, and is widely recognized as a sensitive model for studying the responses of oxygen-sensitive molecular and cellular processes. This study uses the rotating wall vessel bioreactor (RWV) designed at NASA to simulate micro-g. Gene expression in PC12 cells in response to micro-g was analyzed by DNA microarray technology. The microarray analysis of PC12 cells cultured for 4 days under simulated micro-g under standardized oxygen environment conditions revealed more than 100 genes whose expression levels were changed at least twofold (up-regulation of 65 genes and down-regulation of 39 genes) compared with those from cells in the unit gravity (unit-g) control. This study observed that genes involved in the oxidoreductase activity category were most significantly differentially expressed under micro-g conditions. Also, known oxidation-sensitive transcription factors such as hypoxia-inducible factor-2α, c-myc, and the peroxisome proliferator-activated receptor-γ were changed significantly. Our initial results from the gene expression microarray studies may provide a context in which to evaluate the effect of varying oxygen environments on the background of differential gene regulation of biological processes under variable gravity conditions. © 2006 COSPAR.

引用

页码：1168 / 1176

页数：8

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