Purification and properties of a new l-sorbose dehydrogenase accelerative protein from bacillus megaterium bred by ion-beam implantation

被引：11

作者：

Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China ^{[1
]}

机构：

[1] Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences

来源：

Plasma Sci. Technol. | 2008年 / 3卷 / 398-402期

关键词：

Bacillus megaterium BM302; Counterpart protein; Ion beam implantation; Two-step fermentation of Vc;

D O I：

10.1088/1009-0630/10/3/27

中图分类号：

学科分类号：

摘要：

Bacillus megaterium BM302 bred by ion-beam implantation produces L-sorbose dehydrogenase accelerative protein (SAP) to accelerate the activity of L-sorbose dehydrogenase (SDH) of Gluconobacter oxydans in the 2-keto-L-gulonic acid (2KLG) fermentation from L-sorbose by the mixed culture of B. megaterium BM302 and G. oxydans. The SAP purified by three chromatographic steps gave 35-fold purification with a yield of 13% and a specific activity of 5.21 units/mg protein. The molecular weight of the purified SAP was about 58 kDa. The SDH accelerative activity of SAP at pH 7 and 50°C was the highest. Additionally, it retained 60% activity at a pH range of 6.5 ∼ 10 and was stable at 20°C ∼ 60°C. After 0.32-unit SAP was added to the single cultured G. oxydans strains, the SDH activity was apparently accelerated and the 2KLG yield of GO29, GO112, G0 and GI13 was enhanced 2.1, 3.3, 3.5 and 2.9 folds respectively over that of the strains without the addition of SAP.

引用

页码：398 / 402

页数：4

共 18 条

[1]

Lu S., Huang S., Yu J., Eng. Technol. Chem. Reaction, 1, (1985)

[2]

Kieslich K., Present State of Biotechnological Production of Pharmaceuticals, (1984)

[3]

Hoshino T., Sugisawa T., Tazoe, Al E., Agric. Biol. Chem., 54, (1990)

[4]

Sugisawa T., Hoshino T., Masuda S., Al E., Agric. Biol. Chem., 54, (1990)

[5]

Sugisawa T., Hoshino T., Nomura S., Al E., Agric. Biol. Chem., 55, (1991)

[6]

Shinjoh M., Setoguchi Y., Hoshino T., Al E., Agric. Biol. Chem., 54, (1990)

[7]

Hao L., Chen H.Q., Cao G.H., Al E., Chin. J. Apply. Environ. Biol., 8, (2002)

[8]

Nogami I., Shirafuji H., Oka M., Al E., (1987)

[9]

Feng S., Sun C.B., Zhang Z.Z., Al E., J. Microbiology., 8, (1998)

[10]

Hao L., Feng S., Zhang C.G., Al E., Chin. J. Appl. Environ. Biol., 7, (2001)

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