Optimization of culture conditions for bacterial cellulose production by Acetobacter sp. 4B-2

One of the bacterial cellulose (BC) application problems in industry is its low productivity. So, the researchers have tried to increase the productivity of bacterial cellulose using various biochemical. In this study, production of BC using Acetobacter sp. 4B-2 by two categories of carbon sources (monosaccharide and disaccharides) were examined in the modified HS (Hestrin-Shramm) medium by simply replacing D-glucose with other carbon sources. Sucrose gave the highest yield, followed by glucose, xylose and lactose. The highest production of sucrose might be because of the rate of its consumption (80%), which is lower than that of glucose (93.5%). The best yield was achieved by using of 1.5% sucrose. The optimum pH of BC production was 7 and the optimum of temperature for producing BC was 30C. After optimization of culture conditions, production was reached up to 11.98 g L-1 BC. © 2009 Asian Network for Scientific Information.