3D printing cassava starch-ovalbumin intelligent labels: Co-pigmentation effects of gallic acid on anthocyanins

Anthocyanins are often chosen as signal converters of intelligent labels. However, they are degraded by high- temperature oxidation in the process of intelligent label preparation. The color fading seriously affects the sensitivity of color development. In this study, a green 3D printing intelligent label preparation technique was developed, in which gallic acid (GA) was added to a blueberry anthocyanin (BA) solution to enhance the color of the co-pigment to ensure the color sensitivity. The combined effect of GA-BA reduced the fade rate of the anthocyanins from 35.13 % to 26.44 % at 90 degrees C. The printing ink has shear-thinning viscosity characteristics and yield stresses in the range of 500-600 MPa for high-quality printing. Structural analysis revealed that GA-BA co- pigmentation enhanced the interaction between ovalbumin and cassava starch. In addition, the method of 3D printing to prepare labels was conducive to solving the problem of waste in traditional labeling process. The results of freshness testing of sea shrimp proved that labels can be applied to fresh boxes to reflect the freshness of food. We provide a method for enhancing the color of 3D-printed smart ink to prepare intelligent labels with reproducible and customizable batch shapes.