A nanophosphor-based method for selective DNA recovery in synthosomes

被引：21
作者：
International University Bremen, School of Engineering and Science, Bremen, Germany [1 ]
不详 [2 ]
不详 [3 ]
机构：
[1] International University Bremen, School of Engineering and Science, Bremen
[2] Bayer Technology Services GmbH, PT-AS-BP, Leverkusen
[3] Bayer MaterialScience AG, Krefeld
来源：
Biotechnol. J. | 2006年 / 7-8卷 / 828-834期
关键词：
Block copolymer; FhuA; Nanocompartment; Nanophosphors; Synthosome;
D O I：
10.1002/biot.200600042
中图分类号：
学科分类号：
摘要：
A nanocompartment system composed of an ABA triblock copolymer, where A is poly(dimethylsiloxane) and B is poly(2-methyloxazoline), has been developed for selective recovery and detection of DNA. Translocation of TAMRA-labeled complementary primers into the nanocompartment system has been achieved through two deletion mutants (FhuA Δ1-129; FhuA Δ1-160) of the channel protein FhuA. Translocation was monitored by fluorescence resonance energy transfer through hybridization of the TAMRA-labeled primer to the complementary sequence of a nanophosphor-DNA-conjugate, which reduces its half-life (FhuA Δ1-129, 16.0% reduced; FhuA Δ1-160, 39.0% reduced). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：828 / 834
页数：6
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