Antibacterial and cytocompatible silver coating for titanium Boston Keratoprosthesis

被引:1
作者
Gomez, Silvia Gonzalez [1 ,2 ]
Ginebra, Maria-Pau [1 ,2 ,3 ,4 ]
Gil, Francisco Javier [5 ]
Barraquer, Rafael I. [6 ,7 ,8 ]
Manero, Jose Maria [1 ,2 ]
机构
[1] Univ Politecn Catalunya Barcelona Tech UPC, Barcelona East Sch Engn EEBE, Dept Mat Sci & Engn, Biomat Biomech & Tissue Engn Grp, Barcelona, Spain
[2] UPC, Barcelona Res Ctr Multiscale Sci & Engn, EEBE, Barcelona, Spain
[3] Inst Bioengn Catalonia IBEC, Barcelona, Spain
[4] Inst Salud Carlos III, CIBER Bioingn Biomat & Nanomed, Madrid, Spain
[5] Univ Int Catalunya Barcelona, Bioengn Inst Technol, Barcelona, Spain
[6] Ctr Oftalmol Barraquer, Barcelona, Spain
[7] Univ Autonoma Barcelona, Inst Univ Barraquer, Barcelona, Spain
[8] Univ Int Catalunya UIC, Barcelona, Spain
关键词
Boston keratoprosthesis (BKPro); corneal blindness; titanium (Ti); infection; antibacterial properties; electrodeposition; cytotoxicicity; silver deposition; LONG-TERM OUTCOMES; INFECTIOUS ENDOPHTHALMITIS; I KERATOPROSTHESIS; SURFACE; NANOPARTICLES; BIOFILM; ION; CYTOTOXICITY;
D O I
10.3389/fbioe.2024.1421706
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The Boston Keratoprosthesis (BKPro) serves as a medical solution for restoring vision in complex cases of corneal blindness. Comprising a front plate made of polymethylmethacrylate (PMMA) and a back plate of titanium (Ti), this device utilizes the beneficial biomaterial properties of Ti. While BKPro demonstrates promising retention rates, infection emerges as a significant concern that impacts its long-term efficacy. However, limited research exists on enhancement of BKPros through intrinsic infection-preventing mechanisms. In this regard, metal ions, especially the well-known Ag+ ions, are a promising alternative to obtain implants with innate antibacterial properties. However, little information is available about the effects of Ag in corneal tissue, especially within human corneal keratocytes (HCKs). In this work, an electrodeposition treatment using a constant pulse is proposed to attach Ag complexes onto rough Ti surfaces, thus providing antibacterial properties without inducing cytotoxicity. Complete physicochemical characterization and ion release studies were carried out with both control and Ag-treated samples. The possible cytotoxic effects in the short and long term were evaluated in vitro with HCKs. Moreover, the antibacterial properties of the silver-treated surfaces were tested against the gram-negative bacterial strain Pseudomonas aeruginosa and the gram-positive strain Staphylococcus epidermidis, that are common contributors to infections in BKPros. Physicochemical characterization confirmed the presence of silver, predominantly in oxide form, with low release of Ag+ ions. Ag-treated surfaces demonstrated no cytotoxicity and promoted long-term proliferation of HCKs. Furthermore, the silver-treated surfaces exhibited a potent antibacterial effect, causing a reduction in bacterial adhesion and evident damage to the bacterial cell walls of P. aeruginosa and S. epidermidis. The low release of Ag+ ions suggested reactive oxygen species (ROS)-mediated oxidative stress imbalance as the bactericidal mechanism of the silver deposits. In conclusion, the proposed electrodeposition technique confers antibacterial protection to the Ti backplate of BKPro, mitigating implant-threatening infections while ensuring non-cytotoxicity within the corneal tissue.
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页数:16
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共 76 条
[1]  
Ahmad SA, 2020, Materials Science for Energy Technologies, V3, P756, DOI [10.1016/j.mset.2020.09.002, 10.1016/j.mset.2020.09.002, DOI 10.1016/J.MSET.2020.09.002]
[2]   Keratoprostheses for corneal blindness: a review of contemporary devices [J].
Avadhanam, Venkata S. ;
Smith, Helen E. ;
Liu, Christopher .
CLINICAL OPHTHALMOLOGY, 2015, 9 :697-720
[3]   Role of Oxidative and Nitro-Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells [J].
Barcinska, Ewelina ;
Wierzbicka, Justyna ;
Zauszkiewicz-Pawlak, Agata ;
Jacewicz, Dagmara ;
Dabrowska, Aleksandra ;
Inkielewicz-Stepniak, Iwona .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2018, 2018
[4]   Plasma-assisted surface modification of organic biopolymers to prevent bacterial attachment [J].
Bazaka, Kateryna ;
Jacob, Mohan V. ;
Crawford, Russell J. ;
Ivanova, Elena P. .
ACTA BIOMATERIALIA, 2011, 7 (05) :2015-2028
[5]   Infectious endophthalmitis in Boston keratoprosthesis: incidence and prevention [J].
Behlau, Irmgard ;
Martin, Kathryn V. ;
Martin, Jacqueline N. ;
Naumova, Elena N. ;
Cadorette, James J. ;
Sforza, J. Tammy ;
Pineda, Roberto, II ;
Dohlman, Claes H. .
ACTA OPHTHALMOLOGICA, 2014, 92 (07) :e546-e555
[6]   Biocompatibility and biofilm inhibition of N,N-hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials [J].
Behlau, Irmgard ;
Mukherjee, Koushik ;
Todani, Amit ;
Tisdale, Ann S. ;
Cade, Fabiano ;
Wang, Liqiang ;
Leonard, Elizabeth M. ;
Zakka, Fouad R. ;
Gilmore, Michael S. ;
Jakobiec, Frederick A. ;
Dohlman, Claes H. ;
Klibanov, Alexander M. .
BIOMATERIALS, 2011, 32 (34) :8783-8796
[7]   Wettability versus roughness: Multi-scales approach [J].
Belaud, V. ;
Valette, S. ;
Stremsdoerfer, G. ;
Bigerelle, M. ;
Benayoun, S. .
TRIBOLOGY INTERNATIONAL, 2015, 82 :343-349
[8]   Intrinsic cytotoxic effects of fluoroquinolones on human corneal keratocytes and endothelial cells [J].
Bezwada, Padma ;
Clark, Leslie A. ;
Schneider, Susan .
CURRENT MEDICAL RESEARCH AND OPINION, 2008, 24 (02) :419-424
[9]   Evaluation of the microbial reduction efficacy and perception of use of an ozonized water spray disinfection technology [J].
Breda Mascarenhas, Luis Alberto ;
Conceicao dos Santos, Laerte Marlon ;
Oliveira, Fabricia Oliveira ;
Pereira Rodrigues, Leticia de Alencar ;
Freitas Neves, Paulo Roberto ;
Fernandes Moreira, Greta Almeida ;
Bandeira Santos, Alex Alisson ;
Lobato, Gabriela Monteiro ;
Nascimento, Carlos ;
Gerhardt, Marcelo ;
Souza Machado, Bruna Aparecida .
SCIENTIFIC REPORTS, 2022, 12 (01)
[10]   A review of the biomaterials technologies for infection-resistant surfaces [J].
Campoccia, Davide ;
Montanaro, Lucio ;
Arciola, Carla Renata .
BIOMATERIALS, 2013, 34 (34) :8533-8554