Label-free quantitative proteomics of commercial rice beverages reveals the presence of high amounts of exogenous amylases

被引：0

作者：

Battisti, Ilaria ^{[1
,2
,3
,4
]}

Ebinezer, Leonard Barnabas ^{[2
,5
,6
]}

Lomolino, Giovanna ^{[2
]}

Masi, Antonio ^{[2
]}

Arrigoni, Giorgio ^{[1
,3
,4
]}

机构：

[1] Univ Padua, Dept Biomed Sci, Via U Bassi 58-B, I-35131 Padua, Italy

[2] Univ Padua, Dept Agron Food Nat Resources Anim & Environm, Viale Univ 16, I-35020 Legnaro, Italy

[3] Univ Padua, Prote Ctr, Via G Orus 2-B, I-35129 Padua, Italy

[4] Azienda Osped Padova, via G Orus 2-B, I-35129 Padua, Italy

[5] USDA, Edward T Schafer Agr Res Ctr, Northern Crop Sci Lab, 1616 Albrecht Blvd North, Fargo, ND 58102 USA

[6] North Dakota State Univ, Dept Plant Pathol, 306 Walster Hall, Fargo, ND 58105 USA

来源：

FOOD RESEARCH INTERNATIONAL | 2025年 / 200卷

关键词：

Plant-based beverages; Allergens; Amylase; Oryza; Functional drink/beverage; Proteins; ALPHA-AMYLASE; ASTHMA PATIENTS; IDENTIFICATION; EXPRESSION; PROTEINS;

D O I：

10.1016/j.foodres.2024.115464

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Rice milk is known for its wide range of beneficial effects on human health. Despite an increasing inclination towards rice drinks consumption, not much is known regarding the protein composition of commercial rice beverages. Hence, using a label-free quantitative proteomics approach we analyzed 9 different commercially available rice drinks and identified 259 rice proteins and 4 exogenous microbial alpha-amylases. Unexpectedly, exogenous alpha-amylases, when present, were the most abundant in terms of percentage mass. Interestingly, a very strong correlation between the amount of exogenous alpha-amylases and amylase inhibitors was observed and so was the correlation between alpha-amylases and the content of free sugars. We identified 20 potential rice allergens which, together with the exogenous microbial proteins, accounted for 32.3% of the total average percent mass. Taken together, our results have improved the understanding of the protein composition of commercial rice beverages.

引用

页数：12

共 34 条

[1] Utilization of Fermented Rice Milk as a Novel Coagulant for Development of Paneer (Soft Cheese) [J].

Amini, Rasool Khan ;

Islam, Md Zohurul ;

Kitamura, Yutaka ;

Kokawa, Mito .

FOODS, 2019, 8 (08)

[2]

[Anonymous], 2022, Oats Market Is Projected to Reach USD 10.8 Billion by 2032-Comprehensive Research Report by FMI

[3] Bronchial asthma induced by rice [J].

Arai, T ;

Takaya, T ;

Ito, Y ;

Hayakawa, K ;

Toshima, S ;

Shibuya, C ;

Nomura, M ;

Yoshimi, N ;

Shibayama, M ;

Yasuda, Y .

INTERNAL MEDICINE, 1998, 37 (01) :98-101

[4] Protein profile of commercial soybean milks analyzed by label-free quantitative proteomics [J].

Battisti, Ilaria ;

Ebinezer, Leonard Barnabas ;

Lomolino, Giovanna ;

Masi, Antonio ;

Arrigoni, Giorgio .

FOOD CHEMISTRY, 2021, 352

[5] ALLERGIC REACTION AFTER EATING ALPHA-AMYLASE (ASP-O-2)-CONTAINING BREAD [J].

BAUR, X ;

CZUPPON, AB .

ALLERGY, 1995, 50 (01) :85-87

[6]

Bocquet A., 2019, Efficacy and safety

[7] MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification [J].

Cox, Juergen ;

Mann, Matthias .

NATURE BIOTECHNOLOGY, 2008, 26 (12) :1367-1372

[8]

European Union, 2011, Regulation (EU) 1169/2011 of 25 October 2011 on the provision of food information to consumers, amending Regulations (EC) No 1924/2006 and (EC) No 1925/2006 of the European Parliament and of the Council, and repealing Commission Directive 87/250/EEC, Council Directive 90/496/EEC, Commission Directive 1999/10/EC, Directive 2000/13/EC of the European Parliament and of the Council, Commission Directives 2002/67/EC and 2008/5/EC and Commission Regulation (EC) No 608/2004

[9] Quantitative proteomics of heat-treated human cells show an across-the-board mild depletion of housekeeping proteins to massively accumulate few HSPs [J].