A Small-Molecule Photoactivatable Optical Sensor of Transmembrane Potential

被引：0
|
作者：
机构：
[1] Grenier, Vincent
[2] Walker, Alison S.
[3] 1,2,Miller, Evan W.
来源：
Miller, Evan W. (evanwmiller@berkeley.edu) | 1600年 / American Chemical Society卷 / 137期
关键词：
This paper discloses the design; synthesis; and imaging applications of the first member of a new class of SPOTs; small-molecule photoactivatable optical sensors of transmembrane potential. SPOT2.1.Cl features an established voltage-sensitive dye; VoltageFluor2.1.Cl - or VF - capped with a dimethoxy-o-nitrobenzyl (DMNB) caging group to effectively diminish fluorescence of the VF dye prior to uncaging. SPOT2.1.Cl localizes to cell membranes and displays weak fluorescence until photoactivated. Illumination generates the parent VF dye which then optically reports on changes in the membrane voltage. After photoactivation with spatially restricted light; SPOT2.1.Cl-loaded cells display bright; voltage-sensitive fluorescence associated with the plasma membrane; while neighboring cells remain dark. Activated SPOT reports on action potentials in single trials. SPOT can be activated in neuron cell bodies or uncaged in dendrites to enable structural tracing via backfilling of the dye to the soma; followed by functional imaging in the labeled cell. The combination of cellular specificity achieved through spatially defined patterns of illumination; coupled with the fast; sensitive; and noncapacitive voltage sensing characteristics of VF dyes makes SPOT2.1.Cl a useful tool for interrogating both structure and function of neuronal systems. © 2015 American Chemical Society;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
引用
收藏
相关论文
共 50 条
  • [1] A Small-Molecule Photoactivatable Optical Sensor of Transmembrane Potential
    Grenier, Vincent
    Walker, Alison S.
    Miller, Evan W.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (34) : 10894 - 10897
  • [2] A Photoactivatable Small-Molecule Probe for the In Vivo Capture of Polyketide Intermediates
    Kilgour, Samantha L.
    Jenkins, Robert
    Tosin, Manuela
    CHEMISTRY-A EUROPEAN JOURNAL, 2019, 25 (72) : 16511 - 16514
  • [3] The ribosome as a small-molecule sensor
    Bhattacharya, Arunima
    Renault, Thibaud
    Innis, C. Axel
    CURRENT OPINION IN MICROBIOLOGY, 2024, 77
  • [4] SMALL-MOLECULE, BIG POTENTIAL
    JACKSON, A
    ANGOH, G
    CHEMISTRY IN BRITAIN, 1993, 29 (12) : 1046 - 1048
  • [5] Photoactivatable Small-Molecule Inhibitors for Light-Controlled TAM Kinase Activity
    Le Bescont, Julie
    Mouawad, Liliane
    Boddaert, Thomas
    Bombard, Sophie
    Piguel, Sandrine
    CHEMPHOTOCHEM, 2021, 5 (11) : 989 - 994
  • [6] Perspectives on optical biosensor utility in small-molecule screening
    Kaminski, Tim
    Geschwindner, Stefan
    EXPERT OPINION ON DRUG DISCOVERY, 2017, 12 (11) : 1083 - 1086
  • [7] Application of Optical Biosensors in Small-Molecule Screening Activities
    Geschwindner, Stefan
    Carlsson, Johan F.
    Knecht, Wolfgang
    SENSORS, 2012, 12 (04) : 4311 - 4323
  • [8] Small-Molecule Regulators of Autophagy and Their Potential Therapeutic Applications
    Zhou, Mi
    Wang, Renxiao
    CHEMMEDCHEM, 2013, 8 (05) : 694 - 707
  • [9] Small-molecule activation of NAMPT as a potential neuroprotective strategy
    Gu, Xuanyu
    Yao, Hong
    Kwon, Ilmin
    Wang, Gelin
    LIFE MEDICINE, 2022, 1 (03): : 270 - 272
  • [10] A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos
    Morckel, Allison R.
    Lusic, Hrvoje
    Farzana, Laila
    Yoder, Jeffrey A.
    Deiters, Alexander
    Nascone-Yoder, Nanette M.
    DEVELOPMENT, 2012, 139 (02): : 437 - 442
12345