Unravelling the recent advancement in fluorescent probes for detection against reactive sulfur species (RSS) in foodstuffs and cell imaging

Sulfur-containing representative HSO3-/SO32-, H2S, and biothiols (Cys, Hcy, and GSH) present in food items and biological organisms have raised substantial global concerns about food safety due to their reactivity and potential health implications. Adhering to international health standards is essential for these compounds; in particular, plenty of challenges exist in ensuring product quality in the beverage industry. Many fluorescent probes are being employed in various spectroscopic techniques and have developed rapidly to selectively detect sulfur-related species in food products and bio-sensing for cell imaging. This comprehensive review provides a detailed overview of a wide range of fluorescent probes designed using different fluorophores for detecting reactive sulfur species (RSS) using spectroscopic techniques. Additionally, the review explores the detection of RSS components (HSO3-/SO32-, H2S, and biothiols) in food products and cell imaging using different cell lines, highlighting the crucial role of fluorescent probes in swiftly detecting these analytes in both natural and biological contexts. Furthermore, the review discusses future trends and perspectives, emphasizing the on-going progress in detecting these analytes in food products and cell imaging using various fluorescent probes.