Time-resolved dehydration-induced structural changes in an intact bovine cortical bone revealed by solid-state NMR spectroscopy

被引：0
|
作者：
Zhu, Peizhi [1 ]
Xu, Jiadi [1 ,2 ]
Sahar, Nadder [3 ]
Morris, Michael D. [1 ,3 ]
Kohn, David H. [3 ,4 ]
Ramamoorthy, Ayyalusamy [1 ,2 ]
机构：
[1] Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, United States
[2] Department of Biophysics, University of Michigan, Ann Arbor, MI 48109, United States
[3] Department of Biological and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, United States
[4] Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, United States
来源：
Journal of the American Chemical Society | 2009年 / 131卷 / 47期
关键词：
(Figure Presented) Understanding the structure and structural changes of bone; a highly heterogeneous material with a complex hierarchical architecture; continues to be a significant challenge even for high-resolution solid-state NMR spectroscopy. While it is known that dehydration affects mechanical properties of bone by decreasing its strength and toughness; the underlying structural mechanism at the atomic level is unknown. Solid-state NMR spectroscopy; controlled dehydration; and H/D exchange were used for the first time to reveal the structural changes of an intact piece of bovine cortical bone. 1H spectra were used to monitor the dehydration of the bone inside the rotor; and high-resolution 13C chemical shift spectra obtained under magic-angle spinning were used evaluate the dehydration-induced conformational changes in the bone. The experiments revealed the slow denaturation of collagen due to dehydration while the trans-Xaa-Pro conformation in collagen remained unchanged. Our results suggest that glycosaminoglycans in the collagen fiber and mineral interface may chelate with a Ca2+ ion present on the surface of the mineral through sulfate or carboxylate groups. These results provide insights into the role of water molecules in the bone structure and shed light on the relationship between the structure and mechanics of bone. © 2009 American Chemical Society;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：17064 / 17065
相关论文
共 50 条
  • [41] Species-dependent structural polymorphism of Y145Stop prion protein amyloid revealed by solid-state NMR spectroscopy
    Theint Theint
    Philippe S. Nadaud
    Darryl Aucoin
    Jonathan J. Helmus
    Simon P. Pondaven
    Krystyna Surewicz
    Witold K. Surewicz
    Christopher P. Jaroniec
    Nature Communications, 8
  • [42] Species-dependent structural polymorphism of Y145Stop prion protein amyloid revealed by solid-state NMR spectroscopy
    Theint, Theint
    Nadaud, Philippe S.
    Aucoin, Darryl
    Helmus, Jonathan J.
    Pondaven, Simon P.
    Surewicz, Krystyna
    Surewicz, Witold K.
    Jaroniec, Christopher P.
    NATURE COMMUNICATIONS, 2017, 8
  • [43] A NEW METHOD FOR EXAMINING DYNAMICS - TIME-RESOLVED X-RAY SPECTROSCOPY STUDIES OF TRIGGERED REACTIONS USING SOLID-STATE DETECTORS
    CHANCE, MR
    SCHEURING, E
    XIE, A
    MILLER, LM
    LU, Y
    CLAVIN, W
    WU, J
    BIOPHYSICAL JOURNAL, 1994, 66 (02) : A378 - A378
  • [44] Ligand-Induced Conformational Changes of the Multidrug Resistance Transporter EmrE Probed by Oriented Solid-State NMR Spectroscopy
    Gayen, Anindita
    Banigan, James R.
    Traaseth, Nathaniel J.
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (39) : 10321 - 10324
  • [45] Structural diversity of amyloid fibril formed in human calcitonin as revealed by site-directed 13C solid-state NMR spectroscopy
    Naito, A
    Kamihira, M
    Inoue, R
    Saitô, H
    MAGNETIC RESONANCE IN CHEMISTRY, 2004, 42 (02) : 247 - 257
  • [46] Interplay of Structural Factors in Formation of Microphase-Separated or Microphase-Mixed Structures of Polyurethanes Revealed by Solid-State NMR and Dielectric Spectroscopy
    Ostanin, Stepan A.
    Mokeev, Maxim, V
    Pikhurov, Dmitry, V
    Sakhatskii, Aleksandr S.
    Zuev, Vjacheslav V.
    POLYMERS, 2021, 13 (12)
  • [47] Adsorption-Desorption Induced Structural Changes of Cu-MOF Evidenced by Solid State NMR and EPR Spectroscopy
    Jiang, Yijiao
    Huang, Jun
    Kasumaj, Besnik
    Jeschke, Gunnar
    Hunger, Michael
    Mallat, Tamas
    Baiker, Alfons
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (06) : 2058 - +
  • [48] HYDRATION DEHYDRATION-INDUCED CONFORMATIONAL-CHANGES OF AGAROSE, AND KAPPA-CARRAGEENANS AND IOTA-CARRAGEENANS AS STUDIED BY HIGH-RESOLUTION SOLID-STATE C-13-NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY
    SAITO, H
    YOKOI, M
    YAMADA, J
    CARBOHYDRATE RESEARCH, 1990, 199 (01) : 1 - 10
  • [49] Water structural changes in the L and M photocycle intermediates of bacteriorhodopsin as revealed by time-resolved step-scan Fourier transform infrared (FTIR) spectroscopy
    Morgan, Joel E.
    Vakkasoglu, Ahmet S.
    Gennis, Robert B.
    Maeda, Akio
    BIOCHEMISTRY, 2007, 46 (10) : 2787 - 2796
  • [50] Interplay of structural factors in molecular dynamics of microphase-separated or microphase- mixed structures of polyurethanes revealed by solid-state NMR and dielectric spectroscopy
    Ostanin, Stepan A.
    Mokeev, Maxim, V
    Pikhurov, Dmitry, V
    Sakhatskii, Aleksandr S.
    Zuev, Vjacheslav V.
    CHEMICAL PHYSICS IMPACT, 2022, 4
12345